17717-1-AP

antibody from Invitrogen Antibodies

Targeting: MAPRE1 EB1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17717-1-AP

Provider

Invitrogen Antibodies

Product name

EB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAVNVYSTS VTSDNLSRHD MLAWINESLQ LNLTKIEQLC SGAAYCQFMD MLFPGSIALK KVKFQAKLEH EYIQNFKILQ AGFKRMGVDK IIPVDKLVKG KFQDNFEFVQ WFKKFFDANY DGKDYDPVAA RQGQETAVAP SLVAPALNKP KKPLTSSSAA PQRPISTQRT AAAPKAGPGV VRKNPGVGNG DDEAAELMQQ VNVLKLTVED LEKERDFYFG KLRNIELICQ ENEGENDPVL QRIVDILYAT DEGFVIPDEG GPQEEQEEY (1-268 aa encoded by BC106068 )

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of EB1 antibody (17717-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EB1 transfected HeLa cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EB1 (IP:17717-1-AP, 4 ug; Detection:17717-1-AP 1:1000) with Caco-2 cells lysate 1920 ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EB1 (IP:17717-1-AP, 4 ug; Detection:17717-1-AP 1:1000) with mouse thymus tissue lysate 2800 ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with HeLa cells lysate 1200ug.