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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- Ab114244 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- MAPRE1/EB1 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human MAPRE1/EB1 Antibody, Monoclonal (C4), could be used for WB, IHC and so on.ApplicationWB: 0.01-3 µg/mLIHC: 5-20 _g/mLProtein FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 2-268- Antibody clone number
- C4
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- MAPRE1/EB1 Mouse mAb (Ab114244) - Western Blot All lanes: MAPRE1/EB1 Mouse mAb (Ab114244) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 30.1 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- MAPRE1/EB1 Mouse mAb (Ab114244) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 10 µg/mL MAPRE1/EB1 Mouse mAb (Ab114244) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- MAPRE1/EB1 Mouse mAb (Ab114244) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 10 µg/mL MAPRE1/EB1 Mouse mAb (Ab114244) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
