Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF4070 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SOX15 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SOX15 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) SOX3, rhSOX14, and rhSOX21 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O60248
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/β-catenin signaling.
Thu KL, Radulovich N, Becker-Santos DD, Pikor LA, Pusic A, Lockwood WW, Lam WL, Tsao MS
Oncogene 2014 Jan 16;33(3):279-88
Oncogene 2014 Jan 16;33(3):279-88
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SOX15 by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX15 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOX15 in Human iPSK3 cells. SOX15 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.