LS-C312796

antibody from LSBio

Targeting: BUB3 BUB3L

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C312796

Provider

LSBio

Product name

BUB3 Antibody (aa76-90) LS-C312796

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat, Bovine, Guinea Pig, Horse, Porcine, Rabbit, Sheep, Simian

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB of BUB3 antibody. Lane 1: HELA Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4:COLO320 Cell Lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Bub3 antibody, Western blotting All lanes: Anti Bub3 at 0.5ug/ml Lane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 37KD Observed bind size: 37KD

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

BUB3 antibody. ICC: A549 Cell.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

BUB3 antibody. ICC: HELA Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

BUB3 antibody. ICC: A549 Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

BUB3 antibody. ICC: HELA Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

BUB3 antibody. ICC: A549 Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

BUB3 antibody. ICC: HELA Cell.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

BUB3 antibody. IHC(P): Rat Testis Tissue.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

BUB3 antibody. IHC(P): Human Breast Cancer Tissue.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of BUB3 using anti-BUB3 antibody. BUB3 was detected in frozen section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BUB3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of BUB3 using anti-BUB3 antibody. BUB3 was detected in frozen section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BUB3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

BUB3 antibody. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

BUB3 antibody. IHC(P): Human Breast Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of BUB3 using anti-BUB3 antibody. BUB3 was detected in frozen section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BUB3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.