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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX113595 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113595, RRID:AB_2036374
- Product name
- BUB3 antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Nuclear proteomics with XRCC3 knockdown to reveal the development of doxorubicin-resistant uterine cancer.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Nuclear proteomics with XRCC3 knockdown to reveal the development of doxorubicin-resistant uterine cancer.
Chang JF, Lin ST, Hung E, Lu YL, Soon May EW, Lo YW, Chou HC, Chan HL
Toxicological sciences : an official journal of the Society of Toxicology 2014 Jun;139(2):396-406
Toxicological sciences : an official journal of the Society of Toxicology 2014 Jun;139(2):396-406
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE GTX113595 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BUB3 antibody detects BUB3 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody (GTX113595) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 £gg of BUB3 antibody [N1C1] (GTX113595).Western blot analysis was performed using BUB3 antibody [N1C1] (GTX113595).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using BUB3(GTX113595) antibody at 1:100 dilution.
