PAB12997

antibody from Abnova Corporation

Targeting: BUB3 BUB3L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12997

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12997, RRID:AB_10547559

Product name

BUB3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of BUB3.

Storage

Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis.

Kalitsis P, Earle E, Fowler KJ, Choo KH
Genes & development 2000 Sep 15;14(18):2277-82

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The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase.

Taylor SS, Ha E, McKeon F
The Journal of cell biology 1998 Jul 13;142(1):1-11

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The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase.

Roberts BT, Farr KA, Hoyt MA
Molecular and cellular biology 1994 Dec;14(12):8282-91

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of BUB3 in Jurkat cell lysate with BUB3 polyclonal antibody (Cat # PAB12997) at (A) 0.5 and (B) 1 ug/mL .

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunocytochemistry of BUB3 in Jurkat cells with BUB3 polyclonal antibody (Cat # PAB12997) at 10 ug/mL .

Validation comment

Immunocytochemistry