PAB20227

antibody from Abnova Corporation

Targeting: BUB3 BUB3L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20227

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20227, RRID:AB_10968104

Product name

BUB3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant BUB3.

Antigen sequence

YQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEV
QKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTF
ATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAF
SNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDA
ETKPKSP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Liver, Lane 4: Tonsil with BUB3 polyclonal antibody (Cat # PAB20227) at 1:250-1:500 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with BUB3 polyclonal antibody (Cat # PAB20227) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-II

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with BUB3 polyclonal antibody (Cat # PAB20227) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with BUB3 polyclonal antibody (Cat # PAB20227) shows strong nuclear positivity in exocrine glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)