Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-33558 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33558, RRID:AB_2066210
- Product name
- Rabbit Polyclonal Bub3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bub3 Antibody [NBP1-33558] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody diluted by 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Bub3 Antibody [NBP1-33558] - Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 ug of BUB3 antibody [N1C1]. Western blot analysis was performed using BUB3 antibody [N1C1]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-33558] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.