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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [3]
- Immunohistochemistry [5]
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- Product number
- NBP1-88517 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88517, RRID:AB_11007283
- Product name
- Rabbit Polyclonal Bub3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse Bub3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bub3 Antibody [NBP1-88517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bub3 Antibody [NBP1-88517] - Analysis in human cell line HEK 293.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bub3 Antibody [NBP1-88517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
