Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1645 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Bub3 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for BUB3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. BUB3 information: Molecular Weight: 37155 MW; Subcellular Localization: Nucleus. Chromosome, centromere, kinetochore . Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti-Bub3 antibody, PA1645, Western blottingAll lanes: Anti Bub3 (PA1645) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 37KDObserved bind size: 37KD
- Additional image