HPA003601

antibody from Atlas Antibodies

Targeting: BUB3 BUB3L

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA003601

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003601, RRID:AB_1078309

Product name

Anti-BUB3

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human BUB3, Gene description: BUB3 mitotic checkpoint protein, Alternative Gene Names: BUB3L, Validated applications: WB, IHC, ICC, Uniprot ID: O43684, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability

Skowyra A, Allan L, Saurin A, Clarke P
Cell Reports 2018;23(3):852-865

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BUB3 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Sample type

Human

Protocol

Protocol