11504-2-AP

antibody from Proteintech Group

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

Provider product page for 11504-2-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [27]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 11504-2-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11504-2-AP, RRID:AB_2066073
Product name: BubR1 antibody
Antibody type: Polyclonal
Description: KD/KO validated BubR1 antibody (Cat. #11504-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

BUB1B protein structure - 11504-2-AP shown in red.

Submitted references Characteristics of chromosomal instability‑related lncRNAs associated with progression and prognosis in breast cancer.

Chen Q, Chen H, Yang H, Tao Y, Zhu X
Molecular medicine reports 2026 May;33(5)

Targeting the splicing factor CWC22 induces mitotic slippage through repression of BubR1 expression and CDK1 activity in cancer cells.

Yuki R, Saito Y, Nakayama Y
The Journal of biological chemistry 2026 Mar;302(3):111148

Disruption of the NDC80-Nek2 axis suppresses glioblastoma stemness and enhances therapeutic efficacy.

Niu R, Gong R, Wang W, Liu M, Xu CX, Liu HL, Lin L, Jiang T, Yin J
Journal of translational medicine 2026 Feb 9;24(1)

Exploring the carcinogenic potential of bisphenol A in lung adenocarcinoma: molecular mechanisms, key gene insights, and immune microenvironment impacts.

Chen H, Jiang T, Yang Y, Cai G, Jiang Y, Ouyang W
Frontiers in immunology 2025;16:1647807

KIF2C is essential for meiosis and manchette dynamics in male mice.

Harima R, Kishinami M, Hara K, Tanemura K
Frontiers in cell and developmental biology 2025;13:1523593

Chemokines and PI3K/AKT signaling pathway mediate the spontaneously ruptured hepatocellular carcinoma through the regulation of the cell cycle.

Zhang Y, Zhou YL, Xu N, Meng T, Wang ZZ, Pan FM, Zhu LX
Hepatobiliary & pancreatic diseases international : HBPD INT 2025 Oct;24(5):511-526

Kinesin KIF16B Participates in G2/M Transition and Microtubule Dynamics via Aurora A-PLK1 in Oocyte Meiosis.

Li MX, Zhang KH, Zou YJ, Lu PS, Sun SC, Wang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jul 31;39(14):e70854

Significant prognostic value of cell-cycle proteins in early-stage small cell carcinoma of cervix.

Xu Q, Zhou H, Wu J, Chang B, Gu Y, Li H, Cheng X
Scientific reports 2025 Jul 24;15(1):26860

Cyclin B2 facilitates glycolysis and tumor progression in pancreatic ductal adenocarcinoma by promoting histone lactylation.

Ma B, Li X, Ma Z, Yin Z, Gao L, Wang Y, Shi J, Liu X, Chen H
BMC cancer 2025 Dec 7;26(1):63

Propylparaben exposure impairs G2/M and metaphase-anaphase transition during mouse oocyte maturation.

Pan ZN, Zhuang LL, Zhao HS, Yin SY, Chu M, Liu XY, Bao HC
Ecotoxicology and environmental safety 2024 Sep 15;283:116798

SETDB2 interacts with BUBR1 to induce accurate chromosome segregation independently of its histone methyltransferase activity.

Tu Y, Zhang H, Xia J, Zhao Y, Yang R, Feng J, Ma X, Li J
FEBS open bio 2024 Mar;14(3):444-454

Correlation of BUB1 and BUB1B with the development and prognosis of endometrial cancer.

Zhang H, Li Y, Lu H
Scientific reports 2024 Jul 24;14(1):17084

Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients.

Ma Z, Wang Y, Yu Y, Fu F, Zhang Y
Journal of thoracic disease 2024 Feb 29;16(2):911-923

Knockdown of BUB1B Inhibits the Proliferation, Migration, and Invasion of Colorectal Cancer by Regulating the JNK/c-Jun Signaling Pathway.

Zeng Q, Zhang S, He L, Fu Q, Liao L, Chen L, Ding X
Cancer biotherapy & radiopharmaceuticals 2024 Apr;39(3):236-246

Mitotic Dysregulation at Tumor Initiation Creates a Therapeutic Vulnerability to Combination Anti-Mitotic and Pro-Apoptotic Agents for MYCN-Driven Neuroblastoma.

Zhai L, Balachandran A, Larkin R, Seneviratne JA, Chung SA, Lalwani A, Tsubota S, Beck D, Kadomatsu K, Beckers A, Durink K, De Preter K, Speleman F, Haber M, Norris MD, Swarbrick A, Cheung BB, Marshall GM, Carter DR
International journal of molecular sciences 2023 Oct 25;24(21)

Revealing active components and action mechanism of Fritillariae Bulbus against non-small cell lung cancer through spectrum-effect relationship and proteomics.

Liu FJ, Chen XY, Yang J, Zhao Z, Wang QL, Li P, Jiang Y, Li HJ
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Feb;110:154635

Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis.

Mengyan X, Kun D, Xinming J, Yutian W, Yongqian S
Frontiers in pharmacology 2022;13:997842

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I.

Zhou CJ, Wang XY, Dong YH, Wang DH, Han Z, Zhang XJ, Sun QY, Carroll J, Liang CG
Nature communications 2022 Dec 13;13(1):7732

Design, synthesis and antitumor evaluation of novel chiral diaryl substituted azetidin-2-one derivatives as tubulin polymerization inhibitors.

Liang Y, Zhang M, Zhou P, Liu M, Li J, Wang Y
Bioorganic chemistry 2021 Oct;115:105239

The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.

Xu W, Huang M, Guo J, Zhang H, Wang D, Liu T, Liu H, Chen S, Gao P, Mu K
International journal of biological sciences 2020;16(8):1388-1402

Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy.

Zhang YP, Bao ZW, Wu JB, Chen YH, Chen JR, Xie HY, Zhou L, Wu J, Zheng SS
Technology in cancer research & treatment 2020 Jan-Dec;19:1533033820944274

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Yuan F, Jin X, Li D, Song Y, Zhang N, Yang X, Wang L, Zhu WG, Tian C, Zhao Y
Nucleic acids research 2019 Sep 5;47(15):8096-8110

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Zhu ZC, Liu JW, Li K, Zheng J, Xiong ZQ
Oncogene 2018 May;37(22):2936-2952

Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents.

Zhou P, Liang Y, Zhang H, Jiang H, Feng K, Xu P, Wang J, Wang X, Ding K, Luo C, Liu M, Wang Y
European journal of medicinal chemistry 2018 Jan 20;144:817-842

Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells.

Wen C, Chen J, Zhang D, Wang H, Che J, Qin Q, He L, Cai Z, Lin M, Lou Q, Huang L, Chen D, Iwamoto A, Ren D, Wang L, Lan P, Wang J, Liu H, Yang X
Cancer letters 2016 Dec 28;383(2):295-308

Protein phosphatase 4 plays dual roles during cell proliferation.

Huang X, Liu J, Shen T, Meng X, Dou L, Lin Y, Li J
Cell proliferation 2016 Apr;49(2):219-35

SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation.

Yuan YF, Zhai R, Liu XM, Khan HA, Zhen YH, Huo LJ
Molecular reproduction and development 2014 Aug;81(8):712-24

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP(BUB1B antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The BubR1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human BubR1. This antibody recognizes human, mouse antigen. The BubR1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 11504-2-AP.pdf

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