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LearnH00000701-M02
antibody from Novus Biologicals
Targeting: BUB1B
Bub1A, BUBR1, MAD3L, SSK1
Western blot ELISA
Immunocytochemistry Immunohistochemistry Blocking/Neutralizing Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00000701-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000701-M02, RRID:AB_537240
- Product name
- Mouse Monoclonal BubR1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. BUB1B - BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Prognostic implications of securin expression and sub-cellular localization in human breast cancer.
Gurvits N, Repo H, Löyttyniemi E, Nykänen M, Anttinen J, Kuopio T, Talvinen K, Kronqvist P
Cellular oncology (Dordrecht) 2016 Aug;39(4):319-31
Cellular oncology (Dordrecht) 2016 Aug;39(4):319-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi ( Cat # H00000701-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody (M02) clone 2G5 (Cat # H00000701-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - BUB1B monoclonal antibody (M02), clone 2G5 Analysis of BUB1B expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: BubR1 Antibody (2G5) [H00000701-M02] - Detection limit for recombinant GST tagged BUB1B is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.
