RQ5937

antibody from NSJ Bioreagents

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5937

Provider

NSJ Bioreagents

Product name

BubR1 Antibody / BUB1B

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human, Rat

Host

Mouse

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the BubR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) HEK293 and 3) K562 lysate with BubR1 antibody. Predicted molecular weight ~120 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human rectal cancer with BubR1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human rectal cancer with BubR1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat intestine with BubR1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HeLa cells with BubR1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BubR1 antibody.