AF-251-NA

antibody from Novus Biologicals

Targeting: FGF7 KGF

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-251-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal KGF/FGF-7 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human KGF/FGF-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse FGF-7 is observed, less than 20% cross-reactivity with recombinant human (rh) FGF-9 is observed, and less than 10% cross-reactivity with rhFGF-4 and rhFGF-9 is observed.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

250 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Human Multilineage-differentiating Stress-Enduring Cells Exert Pleiotropic Effects to Ameliorate Acute Lung Ischemia-Reperfusion Injury in a Rat Model.

Yabuki H, Wakao S, Kushida Y, Dezawa M, Okada Y
Cell transplantation 2018 Jun;27(6):979-993

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Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.

Nadworny PL, Wang J, Tredget EE, Burrell RE
Journal of inflammation (London, England) 2010 Feb 19;7:13

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Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.

Skibinski G, Elborn JS, Ennis M
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76

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Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.

Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
Investigative ophthalmology & visual science 2006 Mar;47(3):864-72

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Salivary leptin induces increased expression of growth factors in oral keratinocytes.

Gröschl M, Topf HG, Kratzsch J, Dötsch J, Rascher W, Rauh M
Journal of molecular endocrinology 2005 Apr;34(2):353-66

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

KGF/FGF-7 in Human Placenta. KGF/FGF-7 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using Goat Anti-Human KGF/FGF-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by KGF/FGF-7 and Neutralization by Human KGF/FGF-7 Antibody. Recombinant Human KGF/FGF-7 (Catalog # 251-KG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human KGF/FGF-7 (125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human KGF/FGF-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA). The ND₅₀ is typically 6-12 µg/mL.