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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB251-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human KGF/FGF-7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human KGF/FGF-7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) FGF-3, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF acidic, or rhFGF basic is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P21781- Isotype
- IgG
- Antibody clone number
- 29522
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
Induction of keratinocyte growth factor 1 Expression by lipopolysaccharide is regulated by CD-14 and toll-like receptors 2 and 4.
Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
Development (Cambridge, England) 2005 Mar;132(6):1223-34
Development (Cambridge, England) 2005 Mar;132(6):1223-34
Induction of keratinocyte growth factor 1 Expression by lipopolysaccharide is regulated by CD-14 and toll-like receptors 2 and 4.
Putnins EE, Sanaie AR, Wu Q, Firth JD
Infection and immunity 2002 Dec;70(12):6541-8
Infection and immunity 2002 Dec;70(12):6541-8
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by KGF/FGF-7 and Neutralization by Human KGF/FGF-7 Antibody. Recombinant Human KGF/FGF-7 (Catalog # 251-KG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human KGF/FGF-7 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human KGF/FGF-7 Monoclonal Antibody (Catalog # MAB251). The ND50 is typically 0.1-0.5 µg/mL.
