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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-251-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human KGF/FGF-7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human KGF/FGF-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse FGF-7 is observed, less than 20% cross-reactivity with recombinant human (rh) FGF-9 is observed, and less than 10% cross-reactivity with rhFGF-4 and rhFGF-9 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q6FGV5- Isotype
- IgG
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human Multilineage-differentiating Stress-Enduring Cells Exert Pleiotropic Effects to Ameliorate Acute Lung Ischemia-Reperfusion Injury in a Rat Model.
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
Salivary leptin induces increased expression of growth factors in oral keratinocytes.
Yabuki H, Wakao S, Kushida Y, Dezawa M, Okada Y
Cell transplantation 2018 Jun;27(6):979-993
Cell transplantation 2018 Jun;27(6):979-993
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Nadworny PL, Wang J, Tredget EE, Burrell RE
Journal of inflammation (London, England) 2010 Feb 19;7:13
Journal of inflammation (London, England) 2010 Feb 19;7:13
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Skibinski G, Elborn JS, Ennis M
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
Investigative ophthalmology & visual science 2006 Mar;47(3):864-72
Investigative ophthalmology & visual science 2006 Mar;47(3):864-72
Salivary leptin induces increased expression of growth factors in oral keratinocytes.
Gröschl M, Topf HG, Kratzsch J, Dötsch J, Rascher W, Rauh M
Journal of molecular endocrinology 2005 Apr;34(2):353-66
Journal of molecular endocrinology 2005 Apr;34(2):353-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- KGF/FGF-7 in Human Placenta. KGF/FGF-7 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using Goat Anti-Human KGF/FGF-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by KGF/FGF-7 and Neutralization by Human KGF/FGF-7 Antibody. Recombinant Human KGF/FGF-7 (Catalog # 251-KG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human KGF/FGF-7 (125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human KGF/FGF-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA). The ND50 is typically 6-12 µg/mL.
