Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1069 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Resistin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Resistin in direct ELISAs and Western blots. In this format, less than 2% cross-reactivity with recombinant human Resistin and recombinant mouse RELM beta is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q99P87
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis.
Wang F, Gao J, Malisani A, Xi X, Han W, Wan X
BMC biotechnology 2015 Nov 16;15:105
BMC biotechnology 2015 Nov 16;15:105
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Resistin in Mouse Brain. Resistin was detected in perfusion fixed frozen sections of mouse brain (caudate putamen) using 15 µg/mL Mouse Resistin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1069) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.