PA1-1049

antibody from Invitrogen Antibodies

Targeting: RETN ADSF, FIZZ3, RETN1

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-1049

Provider

Invitrogen Antibodies

Product name

Anti-Resistin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-1049 detects recombinant human resistin. PA1-1049 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~7 kDa protein representing a 92 amino acid recombinant mouse resistin. This recombinant protein represents the immature monomeric form of resistin produced in E.coli. In its native form, resistin is a homodimer detectable at ~19 kDa. The PA1-1049 immunogen is a synthetic peptide corresponding to residues C(22) S M E E A I N E R I Q E V A G S L(39) of human resistin. This peptide (Cat. # PEP-218) is available for use in neutralization and control experiments.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Obese subcutaneous adipose tissue impairs human myogenesis, particularly in old skeletal muscle, via resistin-mediated activation of NFκB.

O'Leary MF, Wallace GR, Davis ET, Murphy DP, Nicholson T, Bennett AJ, Tsintzas K, Jones SW
Scientific reports 2018 Oct 18;8(1):15360

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of recombinant human resistin using Product # PA1-1049. Lane 1 is PA1-1049 Lane 2 is PA1-1049 pre-incubated with peptide.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Immunoprecipitation of resistin from obese subcutaneous adipose conditioned medium secretome (OB ACM) improves myogenesis. Resistin was immunoprecipitated from OB ACM using resistin antibody-agarose bead conjugates (OB ACM - resistin IP). IgG isotype antibody control-agarose bead conjugates were used on the same samples as a control (OB ACM). ( A ) Resistin protein is detected by immunoblotting of resistin-antibody lysates but not IgG control lysates following immunopreciptation. Full sized western blot included in Supplementary Fig. 6 . ( B ) Depletion of resistin in OB ACM following resistin immunoprecipitation as determined by ELISA. The dotted horizontal line illustrates the reference value of 1207 pg/mL for the concentration of resistin in NW ACM. ( C ) Subconfluent, commercially available primary human skeletal myoblasts from a female aged 21 yr were switched to either OB ACM differentiation media (OB ACM, n = 4) or to resistin-depleted OB ACM differentiation media (OB ACM Resistin IP, n = 4). Media were renewed every 2 d. At 8 d, myotubes were fixed, immunofluorescence stained for desmin and with DAPI and imaged on an epifluorescence microscope. Nuclear fusion index data are expressed as mean +- SEM values of n = 3 independent experiments. Each independent experiment comprises 15 images taken at 20x magnification. ( D ) Myotube thickness data represent the mean +- SEM of n = 3 independent experiments. Each independent experiment comprises 150 total measuremen