Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 500-P183G-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Resistin Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: SSKTLCSMEE AINERIQEVA GSLIFRAISS IGLECQSVTS RGDLATCPRG FAVTGCTCGS ACGSWDVRAE TTCHCQCAGM DWTGARCCRV QP. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Resistin. Anti-Human Resistin-specific antibody was purified by affinity chromatography employing an immobilized Human Resistin matrix. Sandwich ELISA: To detect Human Resistin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Resistin (500-P183GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Resistin. Western Blot: To detect Human Resistin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Resistin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Goat
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Human resistin protects against endotoxic shock by blocking LPS-TLR4 interaction.
Recombinant human FIZZ3/resistin stimulates lipolysis in cultured human adipocytes, mouse adipose explants, and normal mice.
Jang JC, Li J, Gambini L, Batugedara HM, Sati S, Lazar MA, Fan L, Pellecchia M, Nair MG
Proceedings of the National Academy of Sciences of the United States of America 2017 Nov 28;114(48):E10399-E10408
Proceedings of the National Academy of Sciences of the United States of America 2017 Nov 28;114(48):E10399-E10408
Recombinant human FIZZ3/resistin stimulates lipolysis in cultured human adipocytes, mouse adipose explants, and normal mice.
Ort T, Arjona AA, MacDougall JR, Nelson PJ, Rothenberg ME, Wu F, Eisen A, Halvorsen YD
Endocrinology 2005 May;146(5):2200-9
Endocrinology 2005 May;146(5):2200-9
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human Resistin by Western Blot analysis Resistin Polyclonal Antibody (Product # 500-P183G-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Resistin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human Resistin by Western Blot analysis Resistin Polyclonal Antibody (Product # 500-P183G-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Resistin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human Resistin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of Resistin Polyclonal Antibody (Product # 500-P183G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech Resistin Polyclonal Antibody, Biotin (Product # 500-P183GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Resistin.
