Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AM32709PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti MYOD1
- Antibody type
- Monoclonal
- Antigen
- 14 amino acid peptide whose sequence corresponds to a region that is specific to the MyoD protein and is distal (away) from the homologous helix-loop-helix region of the MyoD protein that is shared by other members of the myogenic bHLH transcription factors.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BC1-C2H8
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml (Lot specific)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blotting with MyoD Antibody Cat.-No AM32709PU-N (Clone BC1-C2H8) used at 2, 3 and 5 µg/ml (in Blotto) on Rh30 rhabdomyosarcoma whole cell lysate and nuclear extract loaded at 10 µg/lane. W= Whole cell lysate, N= Nuclear extract.Secondary Antibody: Peroxidase conjugated Rabbit anti-Mouse Ig used at 1/10,000 dilution in Blotto. Developed by enhanced chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Staining of Rh30 rhabdomyosarcoma cells at using MyoD Antibody Cat.-No AM32709PU-N (Clone BC1-C2H8) at 5 µg/ml is shown. Secondary Antibody: FITC conjugated anti Mouse Ig.