Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- NB100-56511 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56511, RRID:AB_838603
- Product name
- Mouse Monoclonal MyoD Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. In Rh-30, a ~45 kDa band should be observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Prdx6 Plays a Main Role in the Crosstalk Between Aging and Metabolic Sarcopenia.
Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.
The double-edged sword of endoplasmic reticulum stress in uremic sarcopenia through myogenesis perturbation.
MicroRNA-106a-5p Inhibited C2C12 Myogenesis via Targeting PIK3R1 and Modulating the PI3K/AKT Signaling.
Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawal.
Cilia-dependent GLI processing in neural crest cells is required for tongue development.
The homeobox transcription factor Irxl1 negatively regulates MyoD expression and myoblast differentiation.
O(2) regulates skeletal muscle progenitor differentiation through phosphatidylinositol 3-kinase/AKT signaling.
Wnt4 activates the canonical β-catenin pathway and regulates negatively myostatin: functional implication in myogenesis.
Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis.
Skeletal muscle insulin resistance induced by adipocyte-conditioned medium: underlying mechanisms and reversibility.
Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility.
Pacifici F, Della-Morte D, Piermarini F, Arriga R, Scioli MG, Capuani B, Pastore D, Coppola A, Rea S, Donadel G, Andreadi A, Abete P, Sconocchia G, Bellia A, Orlandi A, Lauro D
Antioxidants (Basel, Switzerland) 2020 Apr 17;9(4)
Antioxidants (Basel, Switzerland) 2020 Apr 17;9(4)
Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.
Dogan SA, Cerutti R, Benincá C, Brea-Calvo G, Jacobs HT, Zeviani M, Szibor M, Viscomi C
Cell metabolism 2018 Nov 6;28(5):764-775.e5
Cell metabolism 2018 Nov 6;28(5):764-775.e5
The double-edged sword of endoplasmic reticulum stress in uremic sarcopenia through myogenesis perturbation.
Jheng JR, Chen YS, Ao UI, Chan DC, Huang JW, Hung KY, Tarng DC, Chiang CK
Journal of cachexia, sarcopenia and muscle 2018 Jun;9(3):570-584
Journal of cachexia, sarcopenia and muscle 2018 Jun;9(3):570-584
MicroRNA-106a-5p Inhibited C2C12 Myogenesis via Targeting PIK3R1 and Modulating the PI3K/AKT Signaling.
Li X, Zhu Y, Zhang H, Ma G, Wu G, Xiang A, Shi X, Yang GS, Sun S
Genes 2018 Jul 2;9(7)
Genes 2018 Jul 2;9(7)
Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawal.
Waldemer-Streyer RJ, Reyes-Ordoñez A, Kim D, Zhang R, Singh N, Chen J
NPJ Regenerative medicine 2017;2:16017-
NPJ Regenerative medicine 2017;2:16017-
Cilia-dependent GLI processing in neural crest cells is required for tongue development.
Millington G, Elliott KH, Chang YT, Chang CF, Dlugosz A, Brugmann SA
Developmental biology 2017 Apr 15;424(2):124-137
Developmental biology 2017 Apr 15;424(2):124-137
The homeobox transcription factor Irxl1 negatively regulates MyoD expression and myoblast differentiation.
Chuang HN, Hsiao KM, Chang HY, Wu CC, Pan H
The FEBS journal 2014 Jul;281(13):2990-3003
The FEBS journal 2014 Jul;281(13):2990-3003
O(2) regulates skeletal muscle progenitor differentiation through phosphatidylinositol 3-kinase/AKT signaling.
Majmundar AJ, Skuli N, Mesquita RC, Kim MN, Yodh AG, Nguyen-McCarty M, Simon MC
Molecular and cellular biology 2012 Jan;32(1):36-49
Molecular and cellular biology 2012 Jan;32(1):36-49
Wnt4 activates the canonical β-catenin pathway and regulates negatively myostatin: functional implication in myogenesis.
Bernardi H, Gay S, Fedon Y, Vernus B, Bonnieu A, Bacou F
American journal of physiology. Cell physiology 2011 May;300(5):C1122-38
American journal of physiology. Cell physiology 2011 May;300(5):C1122-38
Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis.
Sun Y, Ge Y, Drnevich J, Zhao Y, Band M, Chen J
The Journal of cell biology 2010 Jun 28;189(7):1157-69
The Journal of cell biology 2010 Jun 28;189(7):1157-69
Skeletal muscle insulin resistance induced by adipocyte-conditioned medium: underlying mechanisms and reversibility.
Sell H, Eckardt K, Taube A, Tews D, Gurgui M, Van Echten-Deckert G, Eckel J
American journal of physiology. Endocrinology and metabolism 2008 Jun;294(6):E1070-7
American journal of physiology. Endocrinology and metabolism 2008 Jun;294(6):E1070-7
Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility.
Dias P, Parham DM, Shapiro DN, Tapscott SJ, Houghton PJ
Cancer research 1992 Dec 1;52(23):6431-9
Cancer research 1992 Dec 1;52(23):6431-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/ml anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: MyoD Antibody (5.8A) [NB100-56511] - Clone 5.8A antibody in human tissues. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent)