Antibody data
- Product number
- MA5-12902
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MYOD Monoclonal Antibody (5.8A)
- Provider product page
- Invitrogen Antibodies - MA5-12902
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-12902 targets MyoD1 in IF, IM, and IP applications and shows reactivity with Chicken, Human, mouse, and Rat samples. The MA5-12902 immunogen is recombinant mouse MyoD1 protein.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5.8A
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
PAX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis.
Kendall GC, Watson S, Xu L, LaVigne CA, Murchison W, Rakheja D, Skapek SX, Tirode F, Delattre O, Amatruda JF
eLife 2018 Jun 5;7
The RNA Surveillance Factor UPF1 Represses Myogenesis via Its E3Â Ubiquitin Ligase Activity.
Feng Q, Jagannathan S, Bradley RK
Molecular cell 2017 Jul 20;67(2):239-251.e6
Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration.
Cohen TV, Kollias HD, Liu N, Ward CW, Wagner KR
The Journal of physiology 2015 Jun 1;593(11):2479-97
Molecular characterization of an EWSR1-POU5F1 fusion associated with a t(6;22) in an undifferentiated soft tissue sarcoma.
Deng FM, Galvan K, de la Roza G, Zhang S, Souid AK, Stein CK
Cancer genetics 2011 Aug;204(8):423-9
MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3.
Hirai H, Verma M, Watanabe S, Tastad C, Asakura Y, Asakura A
The Journal of cell biology 2010 Oct 18;191(2):347-65
CD90-positive cells, an additional cell population, produce laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice.
Fukada S, Yamamoto Y, Segawa M, Sakamoto K, Nakajima M, Sato M, Morikawa D, Uezumi A, Miyagoe-Suzuki Y, Takeda S, Tsujikawa K, Yamamoto H
Experimental cell research 2008 Jan 1;314(1):193-203
Molecular signature of quiescent satellite cells in adult skeletal muscle.
Fukada S, Uezumi A, Ikemoto M, Masuda S, Segawa M, Tanimura N, Yamamoto H, Miyagoe-Suzuki Y, Takeda S
Stem cells (Dayton, Ohio) 2007 Oct;25(10):2448-59
ADP-ribosylation of integrin alpha7 modulates the binding of integrin alpha7beta1 to laminin.
Zhao Z, Gruszczynska-Biegala J, Zolkiewska A
The Biochemical journal 2005 Jan 1;385(Pt 1):309-17
Role of metalloprotease disintegrin ADAM12 in determination of quiescent reserve cells during myogenic differentiation in vitro.
Cao Y, Zhao Z, Gruszczynska-Biegala J, Zolkiewska A
Molecular and cellular biology 2003 Oct;23(19):6725-38
MyoD and myogenin protein expression in skeletal muscles of senile rats.
Dedkov EI, Kostrominova TY, Borisov AB, Carlson BM
Cell and tissue research 2003 Mar;311(3):401-16
Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation.
Holst D, Luquet S, Kristiansen K, Grimaldi PA
Experimental cell research 2003 Aug 1;288(1):168-76
Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation.
Holst D, Luquet S, Kristiansen K, Grimaldi PA
Experimental cell research 2003 Aug 1;288(1):168-76
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of C2C12 (Lane 1). The blots were probed with Anti-MyoD1 Mouse Monoclonal Antibody (Product # MA5-12902, 2.5 µg/mL) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 40 kDa band corresponding to MyoD1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001).The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MyoD1 using MyoD1 Monoclonal Antibody (Product # MA5-12902) on Native Human SW80 Cells.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MyoD1 was done on 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MyoD1 (5.8A) Mouse Monoclonal Antibody (Product # MA5-12902) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
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