Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ABIN953579 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Myogenic Differentiation 1 (MYOD1) (AA 217-247), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the central region (between 217-247aa) of human MYOD1
- Description
- Purified through a Protein A column followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 217-247,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Bone marrow-derived hematopoietic cells undergo myogenic differentiation following a Pax-7 independent pathway.
Methylation status of normal background mucosa is correlated with occurrence and development of neoplasia in the distal colon.
Down-regulation of MyoD by calpain 3 promotes generation of reserve cells in C2C12 myoblasts.
MyoD and E-protein heterodimers switch rhabdomyosarcoma cells from an arrested myoblast phase to a differentiated state.
High-density association study of 383 candidate genes for volumetric BMD at the femoral neck and lumbar spine among older men.
Xynos A, Corbella P, Belmonte N, Zini R, Manfredini R, Ferrari G
Stem cells (Dayton, Ohio) 2010 May;28(5):965-73
Stem cells (Dayton, Ohio) 2010 May;28(5):965-73
Methylation status of normal background mucosa is correlated with occurrence and development of neoplasia in the distal colon.
Hiraoka S, Kato J, Horii J, Saito S, Harada K, Fujita H, Kuriyama M, Takemoto K, Uraoka T, Yamamoto K
Human pathology 2010 Jan;41(1):38-47
Human pathology 2010 Jan;41(1):38-47
Down-regulation of MyoD by calpain 3 promotes generation of reserve cells in C2C12 myoblasts.
Stuelsatz P, Pouzoulet F, Lamarre Y, Dargelos E, Poussard S, Leibovitch S, Cottin P, Veschambre P
The Journal of biological chemistry 2010 Apr 23;285(17):12670-83
The Journal of biological chemistry 2010 Apr 23;285(17):12670-83
MyoD and E-protein heterodimers switch rhabdomyosarcoma cells from an arrested myoblast phase to a differentiated state.
Yang Z, MacQuarrie KL, Analau E, Tyler AE, Dilworth FJ, Cao Y, Diede SJ, Tapscott SJ
Genes & development 2009 Mar 15;23(6):694-707
Genes & development 2009 Mar 15;23(6):694-707
High-density association study of 383 candidate genes for volumetric BMD at the femoral neck and lumbar spine among older men.
Yerges LM, Klei L, Cauley JA, Roeder K, Kammerer CM, Moffett SP, Ensrud KE, Nestlerode CS, Marshall LM, Hoffman AR, Lewis C, Lang TF, Barrett-Connor E, Ferrell RE, Orwoll ES, Zmuda JM, MrOS Research Group
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2009 Dec;24(12):2039-49
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2009 Dec;24(12):2039-49
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane) using MyoD1 antibody Cat.-No AP52799PU-N. This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis with MDA-MB231 cells using MYOD1 antibody Cat.-No. AP52799PU-N, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).