PA5-106727

antibody from Invitrogen Antibodies

Targeting: SERGEF DelGEF, Gnefr

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-106727

Provider

Invitrogen Antibodies

Product name

SERGEF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total SERGEF.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SERGEF in NIH-3T3 cell lysate (left lane: treated with blocking peptide). Samples were incubated with SERGEF polyclonal antibody (Product # PA5-106727).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SERGEF in NIH-3T3 cell lysate (left lane: treated with blocking peptide). Samples were incubated with SERGEF polyclonal antibody (Product # PA5-106727).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SERGEF in NIH/3T3 cells. Samples were incubated with SERGEF polyclonal antibody (Product # PA5-106727).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SERGEF in NIH-3T3 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with SERGEF polyclonal antibody (Product # PA5-106727) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.