PAB20265

antibody from Abnova Corporation

Targeting: LAMTOR2 ENDAP, MAPBPIP, MAPKSP1AP, p14, Ragulator2, ROBLD3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20265

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20265, RRID:AB_10964252

Product name

ROBLD3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ROBLD3.

Antigen sequence

RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGY
GDTDARVTAAIASNIWAAYDRNGNQAFNEDNLKFI
LMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAK
AQALVQYLEEP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with ROBLD3 polyclonal antibody (Cat # PAB20265) at 1:250-1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with ROBLD3 polyclonal antibody (Cat # PAB20265) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)