Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47653 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF366 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ZNF24 and rhZNF206 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with 20 ng/mL recombinant human IL-4 and 50 ng/mL recombinant human GM-CSF for 7 days and 1 µg/mL LPS, 20 ng/mL recombinant human TNF-a, and 20 ng/mL recombinant human IL-1ß/IL-1F2 for last 24 hours were stained with human DC-SCRIPT/ZNF366 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47653), or control antibodyopen histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ZNF366 in Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with 20 ng/mL Recombinant Human IL‚4 and 50 ng/mL Recombinant Human GM‚CSF for 7 days and 1 μg/mL LPS, 20 ng/mL Recombinant Human TNF‚α , and 20 ng/mL Recombinant Human IL‚1β/IL‚1F2 for last 24 hours. Samples were incubated in ZNF366 polyclonal antibody (Product # PA5-47653) or control antibody followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.