AF4707

antibody from R&D Systems

Targeting: ZNF366 FLJ39796

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4707

Provider

R&D Systems

Product name

Human DC-SCRIPT/ZNF366 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human DC-SCRIPT/ZNF366 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ZNF24 and rhZNF206 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q8N895


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

DC-SCRIPT regulates glucocorticoid receptor function and expression of its target GILZ in dendritic cells.

Hontelez S, Karthaus N, Looman MW, Ansems M, Adema GJ
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3172-9

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Dendritic cell-specific transcript: dendritic cell marker and regulator of TLR-induced cytokine production.

Hontelez S, Ansems M, Karthaus N, Zuidscherwoude M, Looman MW, Triantis V, Adema GJ
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 1;189(1):138-45

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DC-SCRIPT: nuclear receptor modulation and prognostic significance in primary breast cancer.

Ansems M, Hontelez S, Looman MW, Karthaus N, Bult P, Bonenkamp JJ, Jansen JH, Sweep FC, Span PN, Adema GJ
Journal of the National Cancer Institute 2010 Jan 6;102(1):54-68

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of DC-SCRIPT/ZNF366 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14⁺ cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days and 1 μg/mL LPS, 20 ng/mL Recombinant Human TNF-alpha (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) for last 24 hours were stained with Human DC-SCRIPT/ZNF366 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4707, filled histogram), or control antibody (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.