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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
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- Product number
- PA1-24891 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPY Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: rat brain.
- Concentration
- Conc. Not Determined
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- ELISA plates (96 well) were coated with various peptides (Columns 1-7) and BSA (Column 8) at 0.5 µg/well. Samples were incubated with a Neuropeptide Y polyclonal antibody (Product # PA1-24891) at a dilution of 1:5000 followed by detection using a goat anti-rabbit IgG-Alkaline Phosphatase secondary antibody at a dilution of 1:5000 with a PNPP substrate; Columns (left to right) 1. Neuropeptide Y (NPY) (sheep) 2. Peptide YY (PYY) (human) 3. Neuropeptide Y (NPY) (porcine) 4. Neuropeptide Y (NPY) (human) 5. Neurokinin B (NKB) (human) 6. Neurokinin A (NKA) (human) 7. Substance P (SP) 8. Albumin, bovine (BSA).
