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Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-19568 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPY Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat mediated antigen retrieval recommended prior to tissue staining. This antibody is predicted to react with sheep, rabbit, chicken, guinea pig, cow, pigeon, pig, zebrafish and rhesus monkey based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.9 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references SerpinA3N is a novel hypothalamic gene upregulated by a high-fat diet and leptin in mice.
Sergi D, Campbell FM, Grant C, Morris AC, Bachmair EM, Koch C, McLean FH, Muller A, Hoggard N, de Roos B, Porteiro B, Boekschoten MV, McGillicuddy FC, Kahn D, Nicol P, Benzler J, Mayer CD, Drew JE, Roche HM, Muller M, Nogueiras R, Dieguez C, Tups A, Williams LM
Genes & nutrition 2018;13:28
Genes & nutrition 2018;13:28
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig. 2 a Representative in situ autoradiograph of a C57BL/6J mouse brain section after 4 weeks of HFD showing serpinA3N expression in the arcuate nuclei (ARC), the ventromedial nuclei of the hypothalamus (VMH), the dorsomedial nuclei of the hypothalamus (DMH) and the lateral hypothalamus (LH). b - e Representative immunostaining of alpha 1 AC (brown) and b GFAP, c Iba1, d AgRP and e NPY (all blue) in the arcuate nuclei after 1 week of HFD. Bar = 20 mum
