12345-1-AP

antibody from Proteintech Group

Targeting: DYNC1H1 CMT2O, DHC1, DNCH1, Dnchc1, DNCL, DNECL, HL-3, p22

Provider product page for 12345-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [67]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 12345-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12345-1-AP, RRID:AB_2261765
Product name: DYNC1H1 antibody
Antibody type: Polyclonal
Description: KD/KO validated DYNC1H1 antibody (Cat. #12345-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, zebrafish, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat, Zebrafish
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

DYNC1H1 protein structure - 12345-1-AP shown in red.

Submitted references iPEX enables micrometre-resolution deep spatial proteomics via tissue expansion.

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Nature 2026 Jan;649(8096):505-514

Glucagon secretion by pancreatic alpha-cells requires an intact tubulin-cytoskeleton-primary cilium axis.

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Super-resolution microscopy reveals a Rab6a-dependent trafficking hub for rhodopsin at the mammalian rod photoreceptor Golgi.

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bioRxiv : the preprint server for biology 2025 Sep 25;

Super-resolution microscopy reveals a Rab6a-dependent trafficking hub for rhodopsin at the mammalian rod photoreceptor Golgi.

Hekmatara M, Thompson SL, Haggerty KN, Hagen S, Brothers BA, Daniels B, Luxardi G, Moshiri A, Deng WT, Robichaux MA
Biology open 2025 Nov 15;14(11)

The Balbiani body is formed by microtubule-controlled molecular condensation of Buc in early oogenesis.

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The Journal of biological chemistry 2025 Apr;301(4):108343

Patient-specific mutation of Dync1h1 in mice causes brain and behavioral deficits.

Ramos RL, De Heredia MMB, Zhang Y, Stout RF, Tindi JO, Wu L, Schwartz GJ, Botbol YM, Sidoli S, Poojari A, Rakowski-Anderson T, Shafit-Zagardo B
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An initial HOPS-mediated fusion event is critical for autophagosome transport initiation from the axon terminal.

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Autophagy 2024 Oct;20(10):2275-2296

KIF1C facilitates retrograde transport of lysosomes through Hook3 and dynein.

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Nesprin-2 coordinates opposing microtubule motors during nuclear migration in neurons.

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Deciphering ER stress-unfolded protein response relationship by visualizing unfolded proteins in the ER.

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Super-resolution mapping in rod photoreceptors identifies rhodopsin trafficking through the inner segment plasma membrane as an essential subcellular pathway.

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A label free chemoproteomic-based platform to disclose cannabidiol molecular mechanism of action on chronic myelogenous leukemia cancer cells.

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Map-1a regulates Sertoli cell BTB dynamics through the cytoskeletal organization of microtubule and F-actin.

Wang L, Yan M, Bu T, Wu X, Li L, Silvestrini B, Sun F, Cheng CY, Chen H
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FMRP Long-Range Transport and Degradation Are Mediated by Dynlrb1 in Sensory Neurons.

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Molecular & cellular proteomics : MCP 2023 Nov;22(11):100653

Non-synonymous, synonymous, and non-coding nucleotide variants contribute to recurrently altered biological processes during retinoblastoma progression.

Stachelek K, Harutyunyan N, Lee S, Beck A, Kim J, Xu L, Berry JL, Nagiel A, Reynolds CP, Murphree AL, Lee TC, Aparicio JG, Cobrinik D
Genes, chromosomes & cancer 2023 May;62(5):275-289

Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity.

He S, Gillies JP, Zang JL, Córdoba-Beldad CM, Yamamoto I, Fujiwara Y, Grantham J, DeSantis ME, Shibuya H
Nature communications 2023 Mar 27;14(1):1715

Phase separation of α-crystallin-GFP protein and its implication in cataract disease.

Shi J, Zhu YX, Huang RY, Bai SM, Zheng YX, Zheng J, Xia ZX, Wang YL
Scientific reports 2023 Mar 24;13(1):4832

A conditional null allele of Dync1h1 enables targeted analyses of dynein roles in neuronal length sensing.

Di Pizio A, Marvaldi L, Birling MC, Okladnikov N, Dupuis L, Fainzilber M, Rishal I
Journal of cell science 2023 Mar 1;136(5)

A dominant negative mitofusin causes mitochondrial perinuclear clusters because of aberrant tethering.

Sloat SR, Hoppins S
Life science alliance 2023 Jan;6(1)

Kinesin-1 Regulates Endocytic Trafficking of Classical Swine Fever Virus along Acetylated Microtubules.

Lou JX, Liu YY, Bai JS, Cheng Y, Zhang J, Liu CC, Zhou B
Journal of virology 2023 Jan 31;97(1):e0192922

Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development.

Shen M, Sirois CL, Guo Y, Li M, Dong Q, Méndez-Albelo NM, Gao Y, Khullar S, Kissel L, Sandoval SO, Wolkoff NE, Huang SX, Xu Z, Bryan JE, Contractor AM, Korabelnikov T, Glass IA, Doherty D, Birth Defects Research Laboratory, Levine JE, Sousa AMM, Chang Q, Bhattacharyya A, Wang D, Werling DM, Zhao X
Neuron 2023 Dec 20;111(24):3988-4005.e11

HEATR5B associates with dynein-dynactin and promotes motility of AP1-bound endosomal membranes.

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The EMBO journal 2023 Dec 1;42(23):e114473

Mapping rhodopsin trafficking in rod photoreceptors with quantitative super-resolution microscopy.

Haggerty KN, Eshelman SC, Sexton LA, Frimpong E, Rogers LM, Agosto MA, Robichaux MA
bioRxiv : the preprint server for biology 2023 Apr 21;

Structuring and validating a prognostic model for low-grade gliomas based on the genes for plasma membrane tension.

Li J, Lv F, Jin T
Frontiers in neurology 2022;13:1024869

A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes.

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Molecular & cellular proteomics : MCP 2022 Nov;21(11):100418

The mechanism of acentrosomal spindle assembly in human oocytes.

Wu T, Dong J, Fu J, Kuang Y, Chen B, Gu H, Luo Y, Gu R, Zhang M, Li W, Dong X, Sun X, Sang Q, Wang L
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361

GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

Bellio C, Emperador M, Castellano P, Gris-Oliver A, Canals F, Sánchez-Pla A, Zamora E, Arribas J, Saura C, Serra V, Tabernero J, Littlefield BA, Villanueva J
Cancers 2022 May 23;14(10)

Rac1, the actin cytoskeleton and microtubules are key players in clathrin-independent endophilin-A3-mediated endocytosis.

Tyckaert F, Zanin N, Morsomme P, Renard HF
Journal of cell science 2022 Jul 15;135(14)

Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Kang Y, Yin S, Liu J, Jiang Y, Huang Z, Chen L, Shao L
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Mechanism of spindle pole organization and instability in human oocytes.

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Science (New York, N.Y.) 2022 Feb 11;375(6581):eabj3944

Arf-like Protein 2 (ARL2) Controls Microtubule Neogenesis during Early Postnatal Photoreceptor Development.

Gerstner CD, Reed M, Dahl TM, Ying G, Frederick JM, Baehr W
Cells 2022 Dec 30;12(1)

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Zhou C, Wu Z, Du W, Que H, Wang Y, Ouyang Q, Jian F, Yuan W, Zhao Y, Tian R, Li Y, Chen Y, Gao S, Wong CCL, Rong Y
Nature cell biology 2022 Apr;24(4):497-512

MIRO2 Regulates Prostate Cancer Cell Growth via GCN1-Dependent Stress Signaling.

Furnish M, Boulton DP, Genther V, Grofova D, Ellinwood ML, Romero L, Lucia MS, Cramer SD, Caino MC
Molecular cancer research : MCR 2022 Apr 1;20(4):607-621

Effect of conditional deletion of cytoplasmic dynein heavy chain DYNC1H1 on postnatal photoreceptors.

Dahl TM, Reed M, Gerstner CD, Ying G, Baehr W
PloS one 2021;16(3):e0248354

Conditional Deletion of Cytoplasmic Dynein Heavy Chain in Postnatal Photoreceptors.

Dahl TM, Reed M, Gerstner CD, Baehr W
Investigative ophthalmology & visual science 2021 Nov 1;62(14):23

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Wang D, Zhang H, Zeng M, Tang X, Zhu X, Guo Y, Qi L, Xie Y, Zhang M, Chen D
Aging 2021 Mar 26;13(7):10240-10274

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Wu X, Sheng H, Wang L, Xia P, Wang Y, Yu L, Lv W, Hu J
Aging 2021 Mar 26;13(7):10034-10057

Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells.

Pedicini L, Wiktor SD, Simmons KJ, Money A, McKeown L
Scientific reports 2021 Feb 18;11(1):4054

A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.

Kesisova IA, Robinson BP, Spiliotis ET
The Journal of cell biology 2021 Feb 1;220(2)

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.

Kumari A, Kumar C, Pergu R, Kumar M, Mahale SP, Wasnik N, Mylavarapu SVS
The Journal of cell biology 2021 Dec 6;220(12)

DYNC1H1 regulates NSCLC cell growth and metastasis by IFN-γ-JAK-STAT signaling and is associated with an aberrant immune response.

Pan H, Chai W, Liu X, Yu T, Sun L, Yan M
Experimental cell research 2021 Dec 1;409(1):112897

Microfilaments and microtubules alternately coordinate the multi-step endosomal trafficking of Classical Swine Fever Virus.

Cheng Y, Lou JX, Liu CC, Liu YY, Chen XN, Liang XD, Zhang J, Yang Q, Go YY, Zhou B
Journal of virology 2021 Apr 26;95(10)

A Conserved Role for Vezatin Proteins in Cargo-Specific Regulation of Retrograde Axonal Transport.

Spinner MA, Pinter K, Drerup CM, Herman TG
Genetics 2020 Oct;216(2):431-445

Transgelin-2 and phosphoregulation of the LIC2 subunit of dynein govern mitotic spindle orientation.

Sharma A, Dagar S, Mylavarapu SVS
Journal of cell science 2020 Jun 23;133(12)

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Kodani A, Kenny C, Lai A, Gonzalez DM, Stronge E, Sejourne GM, Isacco L, Partlow JN, O'Donnell A, McWalter K, Byrne AB, Barkovich AJ, Yang E, Hill RS, Gawlinski P, Wiszniewski W, Cohen JS, Fatemi SA, Baranano KW, Sahin M, Vossler DG, Yuskaitis CJ, Walsh CA
Neuron 2020 Apr 22;106(2):246-255.e6

Enhancing the retrograde axonal transport by curcumin promotes autophagic flux in N2a/APP695swe cells.

Liang J, Zhou F, Xiong X, Zhang X, Li S, Li X, Gao M, Li Y
Aging 2019 Sep 6;11(17):7036-7050

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

So C, Seres KB, Steyer AM, Mönnich E, Clift D, Pejkovska A, Möbius W, Schuh M
Science (New York, N.Y.) 2019 Jun 28;364(6447)

Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.

Wang Q, Tian J, Chen H, Du H, Guo L
Neurobiology of disease 2019 Jul;127:410-418

Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.

Franchini DM, Lanvin O, Tosolini M, Patras de Campaigno E, Cammas A, Péricart S, Scarlata CM, Lebras M, Rossi C, Ligat L, Pont F, Arimondo PB, Laurent C, Ayyoub M, Despas F, Lapeyre-Mestre M, Millevoi S, Fournié JJ
Cell reports 2019 Jan 2;26(1):94-107.e7

EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient 3-dimensional cell migration.

Jayatilaka H, Giri A, Karl M, Aifuwa I, Trenton NJ, Phillip JM, Khatau S, Wirtz D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Mar;32(3):1207-1221

Palladin is a novel microtubule-associated protein responsible for spindle orientation.

Zhang X, Chen X, Liu J, Xu X, Zhang Y, Ruan Z, Xie Y, Huang Q, Yin T, Chen Z, Chen S
Scientific reports 2017 Sep 18;7(1):11806

Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons.

Drerup CM, Herbert AL, Monk KR, Nechiporuk AV
eLife 2017 Apr 17;6

Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint.

Mahale SP, Sharma A, Mylavarapu SV
PloS one 2016;11(7):e0159646

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, Inagaki M
The Journal of cell biology 2016 Feb 15;212(4):409-23

Knockdown of Dynamitin in testes significantly decreased male fertility in Drosophila melanogaster.

Wu CH, Zong Q, Du AL, Zhang W, Yao HC, Yu XQ, Wang YF
Developmental biology 2016 Dec 1;420(1):79-89

Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis.

Gholkar AA, Senese S, Lo YC, Capri J, Deardorff WJ, Dharmarajan H, Contreras E, Hodara E, Whitelegge JP, Jackson PK, Torres JZ
Cell cycle (Georgetown, Tex.) 2015;14(7):1116-25

Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

Renard HF, Simunovic M, Lemière J, Boucrot E, Garcia-Castillo MD, Arumugam S, Chambon V, Lamaze C, Wunder C, Kenworthy AK, Schmidt AA, McMahon HT, Sykes C, Bassereau P, Johannes L
Nature 2015 Jan 22;517(7535):493-6

A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region.

Schroeder CM, Ostrem JM, Hertz NT, Vale RD
eLife 2014 Oct 1;3:e03351

Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe.

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Cell death & disease 2014 Mar 27;5(3):e1151

Loss of Sh3gl2/endophilin A1 is a common event in urothelial carcinoma that promotes malignant behavior.

Majumdar S, Gong EM, Di Vizio D, Dreyfuss J, Degraff DJ, Hager MH, Park PJ, Bellmunt J, Matusik RJ, Rosenberg JE, Adam RM
Neoplasia (New York, N.Y.) 2013 Jul;15(7):749-60

Proteome analysis of the sera from Chinese Parkinson's disease patients.

Zhao X, Xiao WZ, Pu XP, Zhong LJ
Neuroscience letters 2010 Jul 26;479(2):175-9

Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development.

Insinna C, Baye LM, Amsterdam A, Besharse JC, Link BA
Neural development 2010 Apr 22;5:12

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP(KIAA0325 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody 12345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 12345-1-AP(KIAA0325 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue