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LearnPA5-51996
antibody from Invitrogen Antibodies
Targeting: DYNC1H1
CMT2O, DHC1, DNCH1, Dnchc1, DNCL, DNECL, HL-3, p22
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- PA5-51996 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DYNC1H1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RSLETCMYDH KTFSEILNRV QKAVDDLNLH SYSNLPIWVN KLDMEIERIL GVRLQAGLRA WTQVLLGQAE DKAEVDMDTD APQVSHKPGG EPKIKNVVHE LRITNQVIYL NPPIEECRYK LYQEMFAWKM VVLSLPRIQS QR Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 99%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DYNC1H1 Polyclonal Antibody (Product # PA5-51996) and ~532 kDa band corresponding to DYNC1H1 was observed in cell and tissue models tested. Whole cell/ tissue extracts (30 µg lysate) of IMR32 (Lane 1), Mouse brain (Lane 2) and Mouse kidney (Lane 3) were electrophoresed using Novex® NuPAGE™ 3-8% Tris-Acetate gel (Product # EA0375BOX). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001). The bot was probed with the primary antibody (0.1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DYNC1H1 in human cerebral cortex and pancreas tissues using DYNC1H1 Polyclonal Antibody (Product # PA5-51996). Corresponding DYNC1H1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DYNC1H1 in human cerebral cortex using DYNC1H1 Polyclonal Antibody (Product # PA5-51996) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DYNC1H1 in human pancreas using DYNC1H1 Polyclonal Antibody (Product # PA5-51996) shows low expression as expected.
