R34899

antibody from NSJ Bioreagents

Targeting: CD209 CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R34899

Provider

NSJ Bioreagents

Product name

DC-SIGN Antibody

Antibody type

Polyclonal

Description

This highly specific DC-SIGN antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human samples.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Storage

Aliquot and store the DC-SIGN antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human bone marrow lysate with DC-SIGN antibody at 0.3ug/ml. Predicted molecular weight ~46kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human A) K562 and B) ThP-1 cell lysate with DC-SIGN antibody. Predicted molecular weight ~46 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of fixed and permeabilized human ThP-1 cells with DC-SIGN antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placental tissue with DC-SIGN antibody (top) and without DC-SIGN antibody (bottom, negative control) at 6ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of fixed and permeabilized human ThP-1 cells with DC-SIGN antibody (blue) at 10ug/ml and naive goat Ig (black).