AP12204c

antibody from Abcepta

Targeting: ITGA5 CD49e, FNRA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP12204c

Provider

Abcepta

Proper citation

Abgent Cat#AP12204c, RRID:AB_10818250

Product name

ITGA5 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of ITGA5 (arrow) using rabbit polyclonal ITGA5 Antibody (Center) (Cat. #AP12204c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ITGA5 gene.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

ITGA5 Antibody (Center) (Cat. #AP12204c) western blot analysis in human uterine tumor and mouse bladder tissue lysates (35ug/lane).This demonstrates the ITGA5 antibody detected the ITGA5 protein (arrow).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

ITGA5 Antibody (Center) (Cat. #AP12204c)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ITGA5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50