PA5-79529

antibody from Invitrogen Antibodies

Targeting: ITGA5 CD49e, FNRA

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-79529

Provider

Invitrogen Antibodies

Product name

CD49e (Integrin alpha 5) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Cell surface integrin α5ß1 clustering negatively regulates receptor tyrosine kinase signaling in colorectal cancer cells via glycogen synthase kinase 3.

Starchenko A, Graves-Deal R, Brubaker D, Li C, Yang Y, Singh B, Coffey RJ, Lauffenburger DA
Integrative biology : quantitative biosciences from nano to macro 2021 Jun 15;13(6):153-166

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (Product # PA5-79529) . Integrin alpha 5 was detected in a section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Integrin alpha 5 antibody (Product # PA5-79529) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (Product # PA5-79529) . Integrin alpha 5 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Integrin alpha 5 antibody (Product # PA5-79529) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of CD49e (Integrin alpha 5) in HT-1080 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with CD49e (Integrin alpha 5) Polyclonal Antibody (Product # PA5-79529) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of CD49e (Integrin alpha 5) in A431 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with CD49e (Integrin alpha 5) Polyclonal Antibody (Product # PA5-79529) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Presence of Integrin alpha5ss1 at lateral cell-cell junctions correlates with increased epithelial organization in vivo. (A) FFPE sections from human colorectal cancer tumors and adjacent normal regions were stained for integrin alpha5 (green), E-Cadherin (red), and DAPI (blue) (B-C) HCA-7-derived SC cells were injected subcutaneously into nude mice and allowed to establish palpable tumors (1 week). Mice were subsequently given P4G11 or vehicle via intraperitoneal injection for 21 days (B) Representative confocal images of SC tumor sections stained with antibody against E-Cadherin (green), Phalloidin (red) and DAPI (blue). Scale bar = 50um. Inset scale bar = 10um. Note appearance of basolateral antimouse secondary antibody binding in P4G11-treated tumors. (C) Representative histology of control and P4G11-treated tumors, scale bar = 500um, inset scale bar = 50uM.