LS-C313140

TRAM, TRAMP

Antibody data

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Validation data

Product number: LS-C313140
Provider: LifeSpan BioSciences, Inc.
Product name: TRAM1 Antibody (aa314-331) LS-C313140
Provider product page: LifeSpan BioSciences, Inc. - LS-C313140
Antibody type: Polyclonal
Description: Immunogen affinity purified
Reactivity: Human, Mouse, Rat, Bovine, Horse, Simian
Host: Rabbit
Storage: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Provider	Type	Product Number
- No reagents -

Supportive validation

Supportive validation

Supportive validation

Submitted by: LifeSpan BioSciences, Inc. (provider)
Main image
Experimental details: IHC analysis of TRAM1 using anti-TRAM1 antibody. TRAM1 was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-TRAM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: LifeSpan BioSciences, Inc. (provider)
Main image
Experimental details: IHC analysis of TRAM1 using anti-TRAM1 antibody. TRAM1 was detected in frozen section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-TRAM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.