- LS-C313140 - LSBio TRAM1 antibody

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Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: WB of TRAM1 antibody. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Kidney Tissue Lysate. Lane 3: 293T Cell Lysate. Lane 4: RAJI Cell Lysate. Lane 5: JURKAT Cell Lysate.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Anti-TRAM1 antibody,WB,Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: 293T Cell Lysate Lane 4: RAJI Cell Lysate Lane 5: JURKAT Cell Lysate Lane 6: Hela Cell Lysate

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Enhanced validation

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of TRAM1 using anti-TRAM1 antibody. TRAM1 was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-TRAM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of TRAM1 using anti-TRAM1 antibody. TRAM1 was detected in frozen section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-TRAM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of TRAM1 using anti-TRAM1 antibody. TRAM1 was detected in frozen section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-TRAM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Supportive validation

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of TRAM1 using anti-TRAM1 antibody. TRAM1 was detected in frozen section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-TRAM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LS-C313140