Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-80181 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRAM1 in Lane 1: rat brain tissue lysate, Lane 2: rat kidney tissue lysate, Lane 3: 293T cell lysate, Lane 4: RAJI cell lysate, Lane 5: JURKAT cell lysate. Sample was incubated with TRAM1 polyclonal antibody (Product # PA5-80181).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRAM1 in different tissue and cell lystates: Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: 293T Cell Lysate, Lane 4: RAJI Cell Lysate, Lane 5: JURKAT Cell Lysate, Lane 6: Hela Cell Lysate. Samples were incubated with NRF1 Polyclonal Antibody (Product # PA5-80181).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TRAM1 in HeLa cells. Sample was incubated with TRAM1 polyclonal antibody (Product # PA5-80181).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRAM1 on paraffin-embedded human lung cancer tissue. Sample was incubated with TRAM1 polyclonal antibody (Product# PA5-80181).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRAM1 on paraffin-embedded rat kidney tissue. Sample was incubated with TRAM1 polyclonal antibody (Product# PA5-80181).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TRAM1 in frozen section of rat brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-TRAM1 antibody (Product # PA5-80181) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TRAM1 in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-TRAM1 antibody (Product # PA5-80181) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.