MA1-91636

antibody from Invitrogen Antibodies

Targeting: CASP3 apopain, CPP32, CPP32B, Yama

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-91636

Provider

Invitrogen Antibodies

Product name

Anti-pro-Caspase 3 Monoclonal Antibody (31A893)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA1-91636 detects pro Caspase 3 from mouse, human samples. MA1-91636 has been successfully used in Western blot applications. The MA1-91636 immunogen is: Recombinant fusion protein (6xHis tagged full-length human Caspase 3).

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

31A893

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of pro Caspase 3 in Jurkat cells treated with 2 uM staurosporine for different time periods using a pro Caspase 3 monoclonal antibody (Product # MA1-91636). Caspase-3 activation is detected in western blots by the presence of Caspase-3 cleavage fragments. These antibodies detect both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. A basal level of endogenous active Caspase-3 may be see in untreated Jurkat cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of pro Caspase 3 in HL60 lysate. Samples were probed with a pro Caspase 3 monoclonal antibody (Product # MA1-91636) at a dilution of 2 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of pro Caspase 3 in normal human breast tissue using a pro Caspase 3 monoclonal antibody (Product # MA1-91636) at a dilution of 5 µg/mL.