Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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Validation data
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- Product number
- PA1-26426 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Active Caspase 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Positive controls of rat brain or foetus, or mouse foetus suggested.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Neuronal apoptosis can be prevented by the combined therapy with melatonin and hypothermia in a neonatal rat model of hypoxic-ischemic encephalopathy.
Role of PPAR-α agonist fenofibrate in the treatment of induced benign prostatic hyperplasia with dysplastic changes in rats.
Toader AM, Hoteiuc O, Bidian C, Oltean DD, Tabaran F, Grad O, Clichici S, Mitrea DR
Medicine and pharmacy reports 2021 Apr;94(2):197-207
Medicine and pharmacy reports 2021 Apr;94(2):197-207
Role of PPAR-α agonist fenofibrate in the treatment of induced benign prostatic hyperplasia with dysplastic changes in rats.
Refaie MMM, Rifaai RA, Zenhom NM
Fundamental & clinical pharmacology 2018 Dec;32(6):617-626
Fundamental & clinical pharmacology 2018 Dec;32(6):617-626
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Caspase 3 was performed by loading Jurkat cells treated with camptothecin (2 µM). Proteins were transferred to a membrane and probed with a Caspase 3 Polyclonal Antibody (Product # PA1-26426).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoexpression of cleavedcaspase3. Immunohistochemical expression of caspase-3 in the prostate sections from (a) group I (control) and (b) group II [fenofibrate (FEN) given group alone] showed negative expression. (c) Group III [benign prostatic hyperplasia (BPH) induced group] showing weak expression in scattered nuclei (arrows). (d) Group IV showing dense expression in the most of the acinar lining epithelium (arrow). (e) Group V (BPH induced group + FEN treatment) showing positive expression in a larger proportion of epithelial cells (arrows) x400. Semiquantitative analysis of cleaved caspase3. (f) Results showed significant increase in cleaved caspase 3 immunoexpression in BPH treated group with FEN (group V) in comparison with BPH (group III) induced group. Moreover, there is highly significant increase of its expression in BPH withdrawal group (group IV) in comparison with (group V) FEN + BPH group.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Immune-histochemical expression for activated caspase 3 (cas3) in the radial cells of the Ammon''s horn CA1 sub region. Groups: IH-group exposed to cerebral ischemia and hypothermia IN- group exposed to cerebral ischemia and hypoxia, in normal temperature conditions MIH-group melatonin-ischemia-hypothermia, MIN-group melatonin-ischemia-normal thermic conditions. HE staining, x 40.