Antibody data
- Antibody Data
- Antigen structure
- References [74]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9188 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Caspase-3/CASP3 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CASPASE 3/CASP3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CASPASE 3/CASP3 information: Molecular Weight: 31608 MW; Subcellular Localization: Cytoplasm; Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway.
Effects of alendronate on cartilage lesions and micro-architecture deterioration of subchondral bone in patellofemoral osteoarthritic ovariectomized rats with patella-baja.
Cytisine-N-methylene-(5,7,4(')-trihydroxy)- isoflavone ameliorates ischemic stroke-induced brain injury in mouse by regulating the oxidative stress and BDNF-Trkb/Akt pathway.
Borneol-Modified Schisandrin B Micelles Cross the Blood-Brain Barrier To Treat Alzheimer's Disease in Aged Mice.
Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production.
Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study.
Involvement of CXCL10 in Neuronal Damage under the Condition of Spinal Cord Injury and the Potential Therapeutic Effect of Nrg1.
An experimental rat model of electric shock injury with isolated electric shock and water conduction: the histopathological changes on the skin and internal organs and the effect on biochemical parameters.
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Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways.
Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line.
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Cryptotanshinone ameliorates MPP(+)-induced oxidative stress and apoptosis of SH-SY5Y neuroblastoma cells: the role of STAT3 in Parkinson's disease.
MiR-153 downregulation alleviates PTSD-like behaviors and reduces cell apoptosis by upregulating the Sigma-1 receptor in the hippocampus of rats exposed to single-prolonged stress.
Propofol induces the apoptosis of neural stem cells via microRNA-9-5p / chemokine CXC receptor 4 signaling pathway.
Circular RNA Rbms1 inhibited the development of myocardial ischemia reperfusion injury by regulating miR-92a/BCL2L11 signaling pathway.
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Low expression of miR-1 promotes osteogenic repair of bone marrow mesenchymal stem cells by targeting TLR1.
Long non-coding RNA ROR confers arsenic trioxide resistance to HepG2 cells by inhibiting p53 expression.
Changes in the intestinal microecology induced by bacillus subtilis inhibit the occurrence of ulcerative colitis and associated cancers: a study on the mechanisms.
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Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism.
Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy.
Hypoxia promotes pulmonary vascular remodeling via HIF-1α to regulate mitochondrial dynamics.
Metabolomic evidence for the therapeutic effect of gentiopicroside in a corticosterone-induced model of depression.
Treatment with MQA, a Derivative of Caffeoylquinic Acid, Provides Neuroprotective Effects against Cerebral Ischemia Through Suppression of the p38 Pathway and Oxidative Stress in Rats.
The Antiapoptosis Effect of Geum japonicum Thunb. var. chinense Extracts on Cerebral Ischemia Reperfusion Injury via PI3K/Akt Pathway.
Protective effects of extracts of Schisandra chinensis stems against acetaminophen-induced hepatotoxicity via regulation of MAPK and caspase-3 signaling pathways.
Inhibition of glioblastoma growth and invasion by (125)I brachytherapy in rat glioma model.
Experimental study on the therapeutic effect and underlining mechanisms of positron in pancreatic cancer cells.
Inhibitory effects of vitamin E on osteocyte apoptosis and DNA oxidative damage in bone marrow hemopoietic cells at early stage of steroid-induced femoral head necrosis.
Vitamin E TPGS modified liposomes enhance cellular uptake and targeted delivery of luteolin: An in vivo/in vitro evaluation.
Dioscin suppresses hepatocellular carcinoma tumor growth by inducing apoptosis and regulation of TP53, BAX, BCL2 and cleaved CASP3.
Smad2 increases the apoptosis of activated human hepatic stellate cells induced by TRAIL.
Puerarin exerts the protective effect against chemical induced dysmetabolism in rats.
Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats.
Effects of PDTC on NF-κB expression and apoptosis in rats with severe acute pancreatitis-associated lung injury.
The effects of acupuncture and traditional Chinese medicines on apoptosis of brain tissue in a rat intracerebral hemorrhage model.
Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning.
Radioprotective activity of neutral polysaccharides isolated from the fruiting bodies of Hohenbuehelia serotina.
Cytotoxicity of polyaniline nanomaterial on rat celiac macrophages in vitro.
Study of the mechanism of sonodynamic therapy in a rat glioma model.
Sodium fluoride induces apoptosis in the kidney of rats through caspase-mediated pathways and DNA damage.
Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway.
Vinyl chloride monomer (VCM) induces high occurrence of neural tube defects in embryonic mouse brain during neurulation.
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Protective effects of exogenous β-hydroxybutyrate on paraquat toxicity in rat kidney.
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Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts.
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Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy.
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Inhibitory effects of vitamin E on osteocyte apoptosis and DNA oxidative damage in bone marrow hemopoietic cells at early stage of steroid-induced femoral head necrosis.
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Vitamin E TPGS modified liposomes enhance cellular uptake and targeted delivery of luteolin: An in vivo/in vitro evaluation.
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Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning.
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Radioprotective activity of neutral polysaccharides isolated from the fruiting bodies of Hohenbuehelia serotina.
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Cytotoxicity of polyaniline nanomaterial on rat celiac macrophages in vitro.
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Study of the mechanism of sonodynamic therapy in a rat glioma model.
Song D, Yue W, Li Z, Li J, Zhao J, Zhang N
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Sodium fluoride induces apoptosis in the kidney of rats through caspase-mediated pathways and DNA damage.
Song GH, Gao JP, Wang CF, Chen CY, Yan XY, Guo M, Wang Y, Huang FB
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Journal of physiology and biochemistry 2014 Sep;70(3):857-68
Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway.
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Vinyl chloride monomer (VCM) induces high occurrence of neural tube defects in embryonic mouse brain during neurulation.
Quan H, Ma T, Zhao X, Zhao B, Liu Y, Li H
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RASSF1A promotes apoptosis and suppresses the proliferation of ovarian cancer cells.
Fu L, Zhang S
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International journal of molecular medicine 2014 May;33(5):1153-60
Protective effects of exogenous β-hydroxybutyrate on paraquat toxicity in rat kidney.
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Biochemical and biophysical research communications 2014 May 16;447(4):666-71
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Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts.
Dong HY, Wang ZF
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Apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes.
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Neural regeneration research 2014 Apr 15;9(8):837-44
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Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction.
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BMP9-induced osteogenetic differentiation and bone formation of muscle-derived stem cells.
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Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs.
Guan YQ, Li Z, Chen J, Tao H, Wang W, Zheng Z, Li L, Liu JM
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Supportive validation
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- Main image
- Experimental details
- Western blot analysis of Caspase3 using anti-Caspase3 antibody (PB9188). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human Caspase3 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase3 antigen affinity purified polyclonal antibody (Catalog # PB9188) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase3 at approximately 39KD. The expected band size for Caspase3 is at 39KD.
- Additional image