35-1600Z

antibody from Invitrogen Antibodies

Targeting: CASP3 apopain, CPP32, CPP32B, Yama

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

35-1600Z

Provider

Invitrogen Antibodies

Product name

Caspase 3 Monoclonal Antibody (4-1-18)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4/1/2018

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

References

Submitted references

Cytokeratin and protein expression patterns in squamous cell carcinoma of the oral cavity provide evidence for two distinct pathogenetic pathways.

Frohwitter G, Buerger H, VAN Diest PJ, Korsching E, Kleinheinz J, Fillies T
Oncology letters 2016 Jul;12(1):107-113

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In vitro cytotoxicity of 4'-OH-tamoxifen and estradiol in human endometrial adenocarcinoma cells HEC-1A and HEC-1B.

Cuevas ME, Lindeman TE
Oncology reports 2015 Jan;33(1):464-70

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The caspase pathway as a possible therapeutic target in experimental pemphigus.

Pacheco-Tovar D, López-Luna A, Herrera-Esparza R, Avalos-Díaz E
Autoimmune diseases 2011 Mar 2;2011:563091

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The effect of Longan seed polyphenols on colorectal carcinoma cells.

Chung YC, Lin CC, Chou CC, Hsu CP
European journal of clinical investigation 2010 Aug;40(8):713-21

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Mechanisms of grape seed procyanidin-induced apoptosis in colorectal carcinoma cells.

Hsu CP, Lin YH, Chou CC, Zhou SP, Hsu YC, Liu CL, Ku FM, Chung YC
Anticancer research 2009 Jan;29(1):283-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Caspase-3 was performed by loading 20 µg of U-87 MG (lane 1), Hep G2 (lane 2), Ntera-2 (lane 3), Jurkat (lane 4) and HEK-293 (lane 5) (Fig. A) and Jurkat (lane 1), Jurkat treated for O/N with 3 uM of Staurosporine (lane 2), HeLa (lane 3), HeLa treated for O/N with 1 uM of Etoposide (lane 4) and HeLa treated for O/N with 3 uM of Staurosporine in (lane 5) (Fig. B) cell lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Caspase-3 was detected at ~32 kDa using Caspase-3 Mouse Monoclonal Antibody (Product # 35-1600Z) at 1-2 µg/mL in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). (Fig. B) Upon treatment with Staurosporine/Etoposide there was a reduction in total Caspase-3.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Caspase-3 was performed by loading 20 µg of whole cell lysates ofU-87 MG (lane 1), Hep G2 (lane 2), Ntera-2 (lane 3), Jurkat (lane 4) and HEK-293 (lane 5) (Fig. a) and HeLa (lane 1), HeLa treated for O/N with 1 uM of Etoposide (lane 2) (Fig. b) using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Caspase-3 was detected at ~32 kDa using Caspase-3 Mouse Monoclonal Antibody (Product # 35-1600Z) at 1-2 µg/mL in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). (Fig. B) Upon treatment with Staurosporine/Etoposide there was a reduction in total Caspase-3.