Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- 35-1600Z - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 3 Monoclonal Antibody (4-1-18)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4/1/2018
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Submitted references Cytokeratin and protein expression patterns in squamous cell carcinoma of the oral cavity provide evidence for two distinct pathogenetic pathways.
In vitro cytotoxicity of 4'-OH-tamoxifen and estradiol in human endometrial adenocarcinoma cells HEC-1A and HEC-1B.
The caspase pathway as a possible therapeutic target in experimental pemphigus.
The effect of Longan seed polyphenols on colorectal carcinoma cells.
Mechanisms of grape seed procyanidin-induced apoptosis in colorectal carcinoma cells.
Frohwitter G, Buerger H, VAN Diest PJ, Korsching E, Kleinheinz J, Fillies T
Oncology letters 2016 Jul;12(1):107-113
Oncology letters 2016 Jul;12(1):107-113
In vitro cytotoxicity of 4'-OH-tamoxifen and estradiol in human endometrial adenocarcinoma cells HEC-1A and HEC-1B.
Cuevas ME, Lindeman TE
Oncology reports 2015 Jan;33(1):464-70
Oncology reports 2015 Jan;33(1):464-70
The caspase pathway as a possible therapeutic target in experimental pemphigus.
Pacheco-Tovar D, López-Luna A, Herrera-Esparza R, Avalos-Díaz E
Autoimmune diseases 2011 Mar 2;2011:563091
Autoimmune diseases 2011 Mar 2;2011:563091
The effect of Longan seed polyphenols on colorectal carcinoma cells.
Chung YC, Lin CC, Chou CC, Hsu CP
European journal of clinical investigation 2010 Aug;40(8):713-21
European journal of clinical investigation 2010 Aug;40(8):713-21
Mechanisms of grape seed procyanidin-induced apoptosis in colorectal carcinoma cells.
Hsu CP, Lin YH, Chou CC, Zhou SP, Hsu YC, Liu CL, Ku FM, Chung YC
Anticancer research 2009 Jan;29(1):283-9
Anticancer research 2009 Jan;29(1):283-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 was performed by loading 20 µg of U-87 MG (lane 1), Hep G2 (lane 2), Ntera-2 (lane 3), Jurkat (lane 4) and HEK-293 (lane 5) (Fig. A) and Jurkat (lane 1), Jurkat treated for O/N with 3 uM of Staurosporine (lane 2), HeLa (lane 3), HeLa treated for O/N with 1 uM of Etoposide (lane 4) and HeLa treated for O/N with 3 uM of Staurosporine in (lane 5) (Fig. B) cell lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Caspase-3 was detected at ~32 kDa using Caspase-3 Mouse Monoclonal Antibody (Product # 35-1600Z) at 1-2 µg/mL in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). (Fig. B) Upon treatment with Staurosporine/Etoposide there was a reduction in total Caspase-3.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 was performed by loading 20 µg of whole cell lysates ofU-87 MG (lane 1), Hep G2 (lane 2), Ntera-2 (lane 3), Jurkat (lane 4) and HEK-293 (lane 5) (Fig. a) and HeLa (lane 1), HeLa treated for O/N with 1 uM of Etoposide (lane 2) (Fig. b) using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Caspase-3 was detected at ~32 kDa using Caspase-3 Mouse Monoclonal Antibody (Product # 35-1600Z) at 1-2 µg/mL in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). (Fig. B) Upon treatment with Staurosporine/Etoposide there was a reduction in total Caspase-3.