Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- TA300366 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA300366, RRID:AB_2290854
- Product name
- Rabbit Monoclonal Antibody against CASP3 (Clone E87)
- Antibody type
- Monoclonal
- Description
- Rabbit Monoclonal Antibody against CASP3 (Clone E87)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CASP3
- Isotype
- IgG
- Antibody clone number
- E87
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Methyl gallate isolated from Spondias pinnata exhibits anticancer activity against human glioblastoma by induction of apoptosis and sustained extracellular signal-regulated kinase 1/2 activation.
Chaudhuri D, Ghate NB, Singh SS, Mandal N
Pharmacognosy magazine 2015 Apr-Jun;11(42):269-76
Pharmacognosy magazine 2015 Apr-Jun;11(42):269-76
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - Caspase 3 antibody [E87]; All lanes : Anti-Caspase 3 [E87] antibody at 1/5000 dilution.Lane 1 : Untreated Jurkat cell lysate.Lane 2 : Jurkat cell lysate treated with Camptothecin.Predicted band size : 32 kDa.Observed band size : 35 kDa .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Caspase 3 antibody [E87]; Ab32351, at a 1/25 dilution, staining Capase 3 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry - Anti-Caspase 3 antibody; Overlay histogram showing Jurkat cells stained with TA300366 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
- Validation comment
- FC