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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324797 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal CASP3(Asp175) Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal CASP3(Asp175) Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CASP3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.33 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of CASP3 (Asp175) Antibody (Cat. #TA324797) in CEM cell line lysates (35ug/lane). CASP3 (arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody (Cat. #TA324797). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- CASP3(Asp175) Antibody (Cat. #TA324797) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP3(Asp175) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- CASP3(Asp175) Antibody (Cat. #TA324797) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
