PAB29334

antibody from Abnova Corporation

Targeting: CASP3 apopain, CPP32, CPP32B, Yama

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29334

Provider

Abnova Corporation

Product name

CASP3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human CASP3.

Antigen sequence

HGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFH
KSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDL
TREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGI
IFGTNGPVDL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with CASP3 polyclonal antibody (Cat# PAB29334) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with CASP3 polyclonal antibody (Cat# PAB29334) under 1-4 ug/mL working concentration shows positivity in mitochondria and nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow with CASP3 polyclonal antibody (Cat# PAB29334) shows strong cytoplasmic positivity in bone marrow poietic cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)