R31602

antibody from NSJ Bioreagents

Targeting: CASP3 apopain, CPP32, CPP32B, Yama

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31602

Provider

NSJ Bioreagents

Product name

Caspase-3 Antibody

Antibody type

Polyclonal

Description

This highly specific Caspase-3 antibody is suitable for use in Western blot/Immunofluorescence/Immunohistochemistry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Caspase-3 antibody and Lane 1: rat liver; 2: rat thymus; 3: SMMC-7721

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Caspase-3 antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) lysate from untreated human Jurkat cells, 2) lysate from Jurkat cells treated with 1 uM staurosporine, 3) lysate from Jurkat cells treated with 2 uM staurosporine and 4) lysate from untreated human HepG2 cell lysate with Caspase-3 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) lysate from untreated human Jurkat cells, 2) lysate from Jurkat cells treated with 1 uM staurosporine and untreated 3) rat thymus, 4) rat C6, 5) mouse NIH 3T3, 6) mouse RAW264.7 and 7) mouse ANA-1 cell lysate with Caspase-3 antibody.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with Caspase-3 antibody (green).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Caspase-3 antibody testing of human lung cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Caspase-3 antibody testing of mouse intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Caspase-3 antibody testing of rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human tonsil tissue with Caspase-3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human K562 cells with Caspase-3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Caspase-3 antibody.