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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AM08377PU-N - Provider product page
- Provider
- OriGene
- Product name
- Caspase 3 (CASP3) (Full length) mouse monoclonal antibody, clone 31A1067, Purified
- Antibody type
- Monoclonal
- Description
- Caspase 3 (CASP3) (Full length) mouse monoclonal antibody, clone 31A1067, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CASP3
- Isotype
- IgG
- Antibody clone number
- 31A1067
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
Submitted references Mutation in a primate-conserved retrotransposon reveals a noncoding RNA as a mediator of infantile encephalopathy.
Cartault F, Munier P, Benko E, Desguerre I, Hanein S, Boddaert N, Bandiera S, Vellayoudom J, Krejbich-Trotot P, Bintner M, Hoarau JJ, Girard M, Génin E, de Lonlay P, Fourmaintraux A, Naville M, Rodriguez D, Feingold J, Renouil M, Munnich A, Westhof E, Fähling M, Lyonnet S, Henrion-Caude A
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 27;109(13):4980-5
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 27;109(13):4980-5
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 in HeLa cells. The antibody detected both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis. Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in Human, Mouse and Rat heart lysate, respectively.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Caspase-3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Validation comment
- IHC
