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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PAB0242 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0242, RRID:AB_1019080
- Product name
- CASP3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant CASP3.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S
Experimental neurology 2004 Oct;189(2):261-79
Experimental neurology 2004 Oct;189(2):261-79
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CASP3. Lysates from Jurkat cells (lane 1) , normal mammary tissue (lane 2) and surgical specimens from three invasive ductal carcinomas (lanes 3-5) were normalized for total protein content (50 ug/lane) and western blotted with CASP3 polyclonal antibody (Cat # PAB0242) . The ~32 kDa pro-CASP3 protein was detected in all samples. Active/cleaved CASP3 was identified in Jurkat (10 kDa small subunit, lane 1) and two ductal carcinomas (14-21 kDa large subunit) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CASP3 expression in formalin-fixed, paraffin-embedded human reactive lymph node. Using CASP3 polyclonal antibody (Cat # PAB0242) at 1 : 2000. Staining is seen in the apoptosis-prone germinal center B lymphocytes of follicles. In constrast little or no staining is seen in the surrounding long-live mantle zone lymphocytes.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CASP3 expression in formalin-fixed, paraffin-embedded human breast ductal carcinoma in situ. Using CASP3 polyclonal antibody (Cat # PAB0242) at 1 : 2000. Staining is seen in the the cancerous ducts, but not in the normal lobulus.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
