H00000836-D01

antibody from Abnova Corporation

Targeting: CASP3 apopain, CPP32, CPP32B, Yama

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000836-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000836-D01, RRID:AB_10629932

Product name

CASP3 MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human CASP3 protein.

Antigen sequence

MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDN
SYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVD
AANLRETFRNLKYEVRNKNDLTREEIVELMRDVSK
EDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKIT
NFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETD
SGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSK
DGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVAT
EFESFSFDATFHAKKQIPCIVSMLTKELYFYH


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CASP3 MaxPab rabbit polyclonal antibody. Western Blot analysis of CASP3 expression in mouse liver.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CASP3 expression in transfected 293T cell line (H00000836-T01) by CASP3 MaxPab polyclonal antibody.Lane 1: CASP3 transfected lysate(31.60 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to CASP3 on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of CASP3 transfected lysate using anti-CASP3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CASP3 MaxPab rabbit polyclonal antibody (D01) (H00000836-D01).

Validation comment

Immunoprecipitation

Protocol

Protocol