Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- M00334-4 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for CASPASE 3/CASP3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IHC, WB. Reactive species: Human, Mouse, Rat CASPASE 3/CASP3 information: Molecular Weight: 31608 MW; Subcellular Localization: Cytoplasm; Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- DGI-3
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Transmembrane 4 L Six Family Member 1 Suppresses Hormone Receptor--Positive, HER2-Negative Breast Cancer Cell Proliferation.
Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway.
Protective effects of mild hypothermia against hepatic injury in rats with acute liver failure.
Downregulation of long non-coding RNA Opa interacting protein 5-antisense RNA 1 inhibits breast cancer progression by targeting sex-determining region Y-box 2 by microRNA-129-5p upregulation.
microRNA-129-5p suppresses Adriamycin resistance in breast cancer by targeting SOX2.
Dexmedetomidine Alleviates Hyperoxia-Induced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation.
IL-10 reduces levels of apoptosis in Toxoplasma gondii-infected trophoblasts.
Inhibitory effects of β,β-dimethylacrylshikonin on hepatocellular carcinoma in vitro and in vivo.
Chen J, Zhu J, Xu SJ, Zhou J, Ding XF, Liang Y, Chen G, Lu HS
Frontiers in pharmacology 2022;13:770993
Frontiers in pharmacology 2022;13:770993
Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway.
Fu X, Mao Q, Zhang B, Lv J, Ping K, Zhang P, Lin F, Zhao J, Feng Y, Yang J, Wang H, Zhang L, Mou Y, Wang S
Journal of natural products 2022 Apr 22;85(4):1147-1156
Journal of natural products 2022 Apr 22;85(4):1147-1156
Protective effects of mild hypothermia against hepatic injury in rats with acute liver failure.
Zhu Y, Xiang T, Hu D, Shi B, Zhang W, Zhang S, Wu X
Annals of hepatology 2019 Sep-Oct;18(5):770-776
Annals of hepatology 2019 Sep-Oct;18(5):770-776
Downregulation of long non-coding RNA Opa interacting protein 5-antisense RNA 1 inhibits breast cancer progression by targeting sex-determining region Y-box 2 by microRNA-129-5p upregulation.
Zeng H, Wang J, Chen T, Zhang K, Chen J, Wang L, Li H, Tuluhong D, Li J, Wang S
Cancer science 2019 Jan;110(1):289-302
Cancer science 2019 Jan;110(1):289-302
microRNA-129-5p suppresses Adriamycin resistance in breast cancer by targeting SOX2.
Zeng H, Wang L, Wang J, Chen T, Li H, Zhang K, Chen J, Zhen S, Tuluhong D, Li J, Wang S
Archives of biochemistry and biophysics 2018 Aug 1;651:52-60
Archives of biochemistry and biophysics 2018 Aug 1;651:52-60
Dexmedetomidine Alleviates Hyperoxia-Induced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation.
Zhang Q, Wu D, Yang Y, Liu T, Liu H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1907-1919
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1907-1919
IL-10 reduces levels of apoptosis in Toxoplasma gondii-infected trophoblasts.
Zhao M, Zhang R, Xu X, Liu Y, Zhang H, Zhai X, Hu X
PloS one 2013;8(2):e56455
PloS one 2013;8(2):e56455
Inhibitory effects of β,β-dimethylacrylshikonin on hepatocellular carcinoma in vitro and in vivo.
Wu YY, Wan LH, Zheng XW, Shao ZJ, Chen J, Chen XJ, Liu LT, Kuang WJ, Tan XS, Zhou LM
Phytotherapy research : PTR 2012 May;26(5):764-71
Phytotherapy research : PTR 2012 May;26(5):764-71
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human stomach, using active + pro Caspase 3 Antibody (M00334-4)CASP3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP3 Antibody (M00334-4)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image