Antibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- M00334-5 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for CASPASE 3/CASP3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Rat CASPASE 3/CASP3 information: Molecular Weight: 31608 MW; Subcellular Localization: Cytoplasm; Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Antibody clone number
- AOE-3
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references HNF1A induces glioblastoma by upregulating EPS8 and activating PI3K/AKT signaling pathway.
Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway.
Effects of alendronate on cartilage lesions and micro-architecture deterioration of subchondral bone in patellofemoral osteoarthritic ovariectomized rats with patella-baja.
Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production.
Effects of Cooling Interventions with Different Target Temperatures on Heat Stroke Rats.
Involvement of the heat shock response (HSR) regulatory pathway in cadmium-elicited cerebral damage.
Rutaecarpine Ameliorates Murine N-Methyl-N'-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis by Sonic Hedgehog Pathway.
Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways.
Effect of Diammonium Glycyrrhizinate in Improving Focal Cerebral Ischemia-Reperfusion Injury in Rats Through Multiple Mechanisms.
Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Sonodynamic therapy in atherosclerosis by curcumin nanosuspensions: Preparation design, efficacy evaluation, and mechanisms analysis.
Tetrandrine attenuates hyperoxia-induced lung injury in newborn rats via NF-κB p65 and ERK1/2 pathway inhibition.
miR-210-3p regulates the proliferation and apoptosis of non-small cell lung cancer cells by targeting SIN3A.
MicroRNA-93 regulates the neurological function, cerebral edema and neuronal apoptosis of rats with intracerebral hemorrhage through TLR4/NF-κB signaling pathway.
Molecular mechanisms underlying nickel nanoparticle induced rat Sertoli-germ cells apoptosis.
Polyamine analogue QMA attenuated ischemic injury in MCAO rats via ERK and Akt activated Nrf2/HO-1 signaling pathway.
TBX3 deficiency accelerates apoptosis in cardiomyoblasts through regulation of P21 expression.
Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Bone marrow mesenchymal stem cell-derived exosomal miR-21 protects C-kit+ cardiac stem cells from oxidative injury through the PTEN/PI3K/Akt axis.
MiR-429 regulates rat liver regeneration and hepatocyte proliferation by targeting JUN/MYC/BCL2/CCND1 signaling pathway.
Estrogen and propofol combination therapy inhibits endoplasmic reticulum stress and remarkably attenuates cerebral ischemia-reperfusion injury and OGD injury in hippocampus.
RASSF1A promotes apoptosis and suppresses the proliferation of ovarian cancer cells.
Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts.
Apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes.
Neuroprotective properties of picroside II in a rat model of focal cerebral ischemia.
Effects of Kang-Jia-Wan, a Chinese medicinal herb officinal, on apoptosis induction in goiter of rats.
Yang G, Su F, Han BX, Su HX, Guo CH, Yu SH, Guan QL, Hou XM
Biochemical pharmacology 2024 May;223:116133
Biochemical pharmacology 2024 May;223:116133
Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway.
Chen W, Zhang K, Cui H, Li J, Yao D, Wang S, Tian X, Kou W, Huang J, Wang H, Wang J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Mar;172:116276
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Mar;172:116276
Effects of alendronate on cartilage lesions and micro-architecture deterioration of subchondral bone in patellofemoral osteoarthritic ovariectomized rats with patella-baja.
Bei M, Zheng Z, Xiao Y, Liu N, Cao X, Tian F, Zhang L, Wu X
Journal of orthopaedic surgery and research 2024 Mar 25;19(1):197
Journal of orthopaedic surgery and research 2024 Mar 25;19(1):197
Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production.
Zhang Y, Gao X, Cao M, Xu H, Liu H, Zhao Q, Zhou EM, Chen Y, Liu B
Poultry science 2024 Apr;103(4):103501
Poultry science 2024 Apr;103(4):103501
Effects of Cooling Interventions with Different Target Temperatures on Heat Stroke Rats.
Wu C, Wang P, Wang B, Nijiati M, Hou M
Journal of inflammation research 2023;16:2345-2355
Journal of inflammation research 2023;16:2345-2355
Involvement of the heat shock response (HSR) regulatory pathway in cadmium-elicited cerebral damage.
Talukder M, Bi SS, Lv MW, Ge J, Zhang C, Li JL
Environmental science and pollution research international 2023 Oct;30(48):106648-106659
Environmental science and pollution research international 2023 Oct;30(48):106648-106659
Rutaecarpine Ameliorates Murine N-Methyl-N'-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis by Sonic Hedgehog Pathway.
He Y, Liu HH, Zhou XL, He TT, Zhang AZ, Wang X, Wei SZ, Li HT, Chen LS, Chang L, Zhao YL, Jing MY
Molecules (Basel, Switzerland) 2023 Aug 28;28(17)
Molecules (Basel, Switzerland) 2023 Aug 28;28(17)
Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways.
Zhang Q, Qian D, Tang DD, Liu J, Wang LY, Chen W, Wu CJ, Peng W
Journal of agricultural and food chemistry 2022 Sep 7;70(35):10782-10793
Journal of agricultural and food chemistry 2022 Sep 7;70(35):10782-10793
Effect of Diammonium Glycyrrhizinate in Improving Focal Cerebral Ischemia-Reperfusion Injury in Rats Through Multiple Mechanisms.
Wang H, Zhang B, Dong W, Li Y, Zhao L, Zhang Y
Dose-response : a publication of International Hormesis Society 2022 Oct-Dec;20(4):15593258221142792
Dose-response : a publication of International Hormesis Society 2022 Oct-Dec;20(4):15593258221142792
Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Sun M, Zhou D, Wu J, Zhou J, Xu J
Marine drugs 2022 Feb 5;20(2)
Marine drugs 2022 Feb 5;20(2)
Sonodynamic therapy in atherosclerosis by curcumin nanosuspensions: Preparation design, efficacy evaluation, and mechanisms analysis.
Jiang L, Wang J, Jiang J, Zhang C, Zhao M, Chen Z, Wang N, Hu D, Liu X, Peng H, Lian M
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2020 Jan;146:101-110
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2020 Jan;146:101-110
Tetrandrine attenuates hyperoxia-induced lung injury in newborn rats via NF-κB p65 and ERK1/2 pathway inhibition.
Jiao B, Tang Y, Liu S, Guo C
Annals of translational medicine 2020 Aug;8(16):1018
Annals of translational medicine 2020 Aug;8(16):1018
miR-210-3p regulates the proliferation and apoptosis of non-small cell lung cancer cells by targeting SIN3A.
Ren J, Li X, Dong H, Suo L, Zhang J, Zhang L, Zhang J
Experimental and therapeutic medicine 2019 Oct;18(4):2565-2573
Experimental and therapeutic medicine 2019 Oct;18(4):2565-2573
MicroRNA-93 regulates the neurological function, cerebral edema and neuronal apoptosis of rats with intracerebral hemorrhage through TLR4/NF-κB signaling pathway.
Shang Y, Dai S, Chen X, Wen W, Liu X
Cell cycle (Georgetown, Tex.) 2019 Nov;18(22):3160-3176
Cell cycle (Georgetown, Tex.) 2019 Nov;18(22):3160-3176
Molecular mechanisms underlying nickel nanoparticle induced rat Sertoli-germ cells apoptosis.
Kong L, Hu W, Gao X, Wu Y, Xue Y, Cheng K, Tang M
The Science of the total environment 2019 Nov 20;692:240-248
The Science of the total environment 2019 Nov 20;692:240-248
Polyamine analogue QMA attenuated ischemic injury in MCAO rats via ERK and Akt activated Nrf2/HO-1 signaling pathway.
Cen J, Zhao N, Huang WW, Liu L, Xie YY, Gan Y, Wang CJ, Ji BS
European journal of pharmacology 2019 Feb 5;844:165-174
European journal of pharmacology 2019 Feb 5;844:165-174
TBX3 deficiency accelerates apoptosis in cardiomyoblasts through regulation of P21 expression.
Cao M, Zhu B, Sun Y, Zhao X, Qiu G, Fu W, Jiang H
Life sciences 2019 Dec 15;239:117040
Life sciences 2019 Dec 15;239:117040
Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Zhong G, Liang R, Yao J, Li J, Jiang T, Liu J, Le Y, Shen C, Long H, Lu H, Ma S, Luo J, Miao Z, Su W, Zheng L, Zhao J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;51(6):2575-2590
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;51(6):2575-2590
Bone marrow mesenchymal stem cell-derived exosomal miR-21 protects C-kit+ cardiac stem cells from oxidative injury through the PTEN/PI3K/Akt axis.
Shi B, Wang Y, Zhao R, Long X, Deng W, Wang Z
PloS one 2018;13(2):e0191616
PloS one 2018;13(2):e0191616
MiR-429 regulates rat liver regeneration and hepatocyte proliferation by targeting JUN/MYC/BCL2/CCND1 signaling pathway.
Zhang C, Chang C, Gao H, Wang Q, Zhang F, Xu C
Cellular signalling 2018 Oct;50:80-89
Cellular signalling 2018 Oct;50:80-89
Estrogen and propofol combination therapy inhibits endoplasmic reticulum stress and remarkably attenuates cerebral ischemia-reperfusion injury and OGD injury in hippocampus.
Xu F, Ma R, Zhang G, Wang S, Yin J, Wang E, Xiong E, Zhang Q, Li Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Dec;108:1596-1606
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Dec;108:1596-1606
RASSF1A promotes apoptosis and suppresses the proliferation of ovarian cancer cells.
Fu L, Zhang S
International journal of molecular medicine 2014 May;33(5):1153-60
International journal of molecular medicine 2014 May;33(5):1153-60
Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts.
Dong HY, Wang ZF
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2014 Apr;26(2):200-7
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2014 Apr;26(2):200-7
Apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes.
Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L
Neural regeneration research 2014 Apr 15;9(8):837-44
Neural regeneration research 2014 Apr 15;9(8):837-44
Neuroprotective properties of picroside II in a rat model of focal cerebral ischemia.
Li Q, Li Z, Xu XY, Guo YL, Du F
International journal of molecular sciences 2010 Nov 16;11(11):4580-90
International journal of molecular sciences 2010 Nov 16;11(11):4580-90
Effects of Kang-Jia-Wan, a Chinese medicinal herb officinal, on apoptosis induction in goiter of rats.
Han Y, Gao L, Sun N, Zhang J, Zhang W, Zhou X, Zhang H, Zhao J
Journal of ethnopharmacology 2009 Apr 21;122(3):533-40
Journal of ethnopharmacology 2009 Apr 21;122(3):533-40
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human tonsil, using Caspase-3 Antibody(M00334-5)CASP3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP3 Antibody (M00334-5)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image